Probe:	Let-7d probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: neural retina	Weak	Regionally restricted	1A	Low expression levels in progenitor cells (neuroblastic layer).
TS19: pigmented retinal epithelium	Absent		1A

Structure	Level	Pattern	Image	Note
TS21: neural retina	Present	Regionally restricted	1B	Expression in all retinal layers; slightly enriched in neuroblastic layer. High expression along apical surface of retina.
TS21: lens epithelium	Strong		1B	Higher expression in lens epithelium.
TS21: lens	Present		1B	Higher expression in bow region. No expression in lens fibers.
TS21: pigmented retinal epithelium	Absent		1B

Structure	Level	Pattern	Image	Note
TS24: eye anterior segment	Strong	Regionally restricted	1C	Highest level of expression in ciliary body.
TS24: neural retina	Present	Regionally restricted	1C	Expression in all retinal layers; slightly enriched in neuroblastic layer. High expression along apical surface of retina.
TS24: pigmented retinal epithelium	Present		1C	Expression in retinal pigment epithelium from E16.
TS24: lens epithelium	Present	Regionally restricted	1C	Higher expression in lens epithelium and bow region.
TS24: lens fiber	Absent		1C

Structure	Level	Pattern	Image	Note
TS27: ciliary body	Strong		1D	Highest level of expression in ciliary body.
TS27: neural retina	Present	Regionally restricted	1D	Expression in all retinal layers; slightly enriched in neuroblastic layer. High expression along apical surface of retina.
TS27: pigmented retinal epithelium	Present		1D
TS27: lens epithelium	Strong		1D	Higher expression in lens epithelium and bow region.
TS27: lens fiber	Absent		1D

Structure	Level	Pattern	Image	Note
TS19: telencephalon ventricular layer	Weak		1E	Low level of expression in ventricular zone progenitors.
TS19: telencephalon	Strong	Regionally restricted	1E	High level of expression in post-mitotic neurons of preplate.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Strong	Regionally restricted	1F	Expression enriched in ventricular zone progenitors.
TS21: neocortex	Present	Regionally restricted	1F	Lower expression in post-mitotic neurons.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Strong	Regionally restricted	1G	Increased expression in ventricular zone progenitors. Particularly high level of expression along apical surface near the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex ventricular zone	Strong	Regionally restricted	1H	Increased expression in ventricular zone progenitors. Particularly high level of expression along apical surface near the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS19: telencephalon ventricular layer	Weak	Regionally restricted	1I	Low levels of expression in early hippocampus primordium ventricular zone progenitors.

Structure	Level	Pattern	Image	Note
TS21: telencephalon ventricular layer	Present	Regionally restricted	1J	Increasingly higher levels of expression in hippocampus ventricular zone from E13.5 onwards.

Structure	Level	Pattern	Image	Note
TS24: hippocampus ventricular layer	Strong		1K	Increasingly higher levels of expression in hippocampus ventricular zone.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong	Regionally restricted	1L	Increasingly higher levels of expression in hippocampus ventricular zone

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Weak	Regionally restricted	1M	Low levels of expression in early ventricular zone progenitors.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		1N	Increasingly higher levels of expression from E13.5 onwards.

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present		1O

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		1P

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present		S1A

Structure	Level	Pattern	Image	Note
TS21: neocortex	Weak		S1B	Expression greatly reduced compared to wild type littermates.

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present		S1C
TS21: retina	Present		S1C

Structure	Level	Pattern	Image	Note
TS21: retina	Present		S1D	Expression comparable to that of wild type littermates.

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Regionally restricted	2A , 2A''	Variable levels of let-7d expression within Pax6-expressing progenitors along apical-basal axis of Pax6 domain. Higher levels of expression in basal region compared to those located more apically.

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present		2B , 2B''

Structure	Level	Pattern	Image	Note
TS21: neocortex	Weak	Regionally restricted	2C , 2C''	Lower levels of expression in phospho-histone H3 expressing cells compared to neighboring cells.

Structure	Level	Pattern	Image	Note
TS21: neocortex	Strong	Regionally restricted	2D	Highest level of expression in region where cells in S-phase are present.

Structure	Level	Pattern	Image	Note
TS19: retina	Present	Regionally restricted	2H	Expression levels vary within retinal progenitors. Higher leevls of expression in more basally located progenitors compared to those located more apically.

Structure	Level	Pattern	Image	Note
TS21: retina	Present	Regionally restricted	S2C	Highest levels of expression in cells residing at apical surface.

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted
TS19: cornea epithelium	Weak
TS19: optic stalk	Weak
TS19: upper rhombic lip	Weak

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Strong
TS21: upper rhombic lip	Strong
TS21: olfactory cortex	Strong	Regionally restricted		Strong expression in piriform cortex.
TS21: epithalamus	Present
TS21: habenula	Present
TS21: olfactory lobe	Present
TS21: preoptic area	Present
TS21: cerebellum	Present	Regionally restricted		Expression in Purkinje cell layer.
TS21: thalamus	Present	Regionally restricted		Moderate expression in dorsal and medial thalamus; weak expression in lateral thalamus.
TS21: cerebellum layer	Weak
TS21: cortical subplate	Weak
TS21: mammillary area	Weak
TS21: cornea epithelium	Trace
TS21: cortical plate	Trace
TS21: optic nerve	Trace
TS21: optic stalk	Trace
TS21: cerebral cortex intermediate zone	Absent
TS21: cerebral cortex marginal layer	Absent

Structure	Level	Pattern	Image	Note
TS24: upper rhombic lip	Very strong
TS24: cerebellum external granule cell layer	Strong
TS24: ganglionic eminence	Strong
TS24: olfactory cortex	Strong
TS24: piriform cortex	Strong
TS24: retrosplenial cortex	Strong
TS24: superior colliculus	Strong
TS24: thalamus	Present	Regionally restricted		Strong expression in medial thalamus; moderate expression in dorsal thalamus; weak expression in lateral thalamus
TS24: anterior hypothalamic nucleus	Moderate
TS24: arcuate nucleus	Moderate
TS24: cerebral cortex subventricular zone	Moderate
TS24: dorsomedial hypothalamic nucleus	Moderate
TS24: epithalamus	Moderate
TS24: habenula	Moderate
TS24: inferior colliculus	Moderate
TS24: lateral geniculate nucleus	Moderate
TS24: preoptic area	Moderate
TS24: neocortex	Present	Regionally restricted		Expression in cortical layers VI, VI and VI.
TS24: telencephalon septum	Present	Regionally restricted		Moderate expression in medial septum. Weak expression in dorso-lateral septum.
TS24: retina layer	Present	Regionally restricted		Expression in ganglion cell layer.
TS24: caudate-putamen	Weak
TS24: cornea	Weak
TS24: cortical subplate	Weak
TS24: entorhinal cortex	Weak
TS24: globus pallidus	Weak
TS24: mammillary area	Weak
TS24: hippocampus	Weak	Regionally restricted		Expression in CA1, CA2 and CA3
TS24: cerebral cortex intermediate zone	Trace
TS24: optic nerve	Trace
TS24: optic stalk	Trace
TS24: cerebral cortex marginal layer	Absent

Structure	Level	Pattern	Image	Note
TS27: amygdaloid nucleus	Very strong
TS27: cerebellum external granule cell layer	Very strong
TS27: cerebral cortex subventricular zone	Very strong
TS27: piriform cortex	Very strong
TS27: telencephalon	Very strong	Regionally restricted		Authors report very strong expression in mitral cell layer. Moderate expression in granular cell layer. Weak expression in glomerular layer.
TS27: accumbens nucleus	Strong
TS27: cerebellum Purkinje cell layer	Strong
TS27: cingulate cortex	Strong
TS27: cortical layer II	Strong
TS27: cortical layer III	Strong
TS27: entorhinal cortex	Strong
TS27: retrosplenial cortex	Strong
TS27: superior colliculus	Strong
TS27: thalamus	Present	Regionally restricted		Strong expression in medial thalamus; moderate expression in dorsal thalamus; weak expression in lateral thalamus.
TS27: anterior hypothalamic nucleus	Moderate
TS27: cortical layer IV	Moderate
TS27: cortical layer V	Moderate
TS27: cortical layer VI	Moderate
TS27: dorsomedial hypothalamic nucleus	Moderate
TS27: epithalamus	Moderate
TS27: habenula	Moderate
TS27: hippocampus CA1	Moderate
TS27: hippocampus CA2	Moderate
TS27: hippocampus CA3	Moderate
TS27: inferior colliculus	Moderate
TS27: lateral geniculate nucleus	Moderate
TS27: preoptic area	Moderate
TS27: retina ganglion cell layer	Moderate
TS27: retina outer nuclear layer	Moderate
TS27: telencephalon septum	Present	Regionally restricted		Moderate expression in dorso-lateral septum; Weak expression in medial septum.
TS27: arcuate nucleus	Weak
TS27: cerebellum vermis	Weak
TS27: cornea	Weak
TS27: dentate gyrus	Weak
TS27: dorsal raphe nucleus	Weak
TS27: globus pallidus	Weak
TS27: periaqueductal grey	Weak
TS27: subiculum	Weak
TS27: fimbria hippocampus	Trace
TS27: mammillary area	Trace
TS27: cerebral cortex marginal zone	Absent
TS27: optic nerve	Absent
TS27: optic stalk	Absent