Antibody:	anti-Bsg
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS20: pigmented retinal epithelium	Strong		1A
TS20: eye anterior segment	Strong	Regionally restricted	1A	Expression is in the pre-ciliary and pre-iris anterior margin of the retina.
TS20: retina	Strong	Regionally restricted	1A	Expression is in the pre-ciliary and pre-iris anterior margin of the retina.
TS20: cornea epithelium	Present		1A
TS20: lens vesicle epithelium	Present		1A
TS20: lens vesicle	Present	Regionally restricted	1A	Expression is in the anterior portion.

Structure	Level	Pattern	Image	Note
TS23: retina	Strong	Regionally restricted	1B	Expression is strongest at the anterior margin of the retina.
TS23: pigmented retinal epithelium	Present		1B	Expression is at both the apical and basal membranes.
TS23: retina nerve fiber layer	Weak		1B

Structure	Level	Pattern	Image	Note
TS26: retina	Very strong	Regionally restricted	2A	Expression was most intense on the tissue continuous with the anterior tip of the neural retina bordering the ciliary-pigmented epithelium. Expression was weak in the invagination.
TS26: ciliary body	Very strong	Regionally restricted	2A	Expression is in the non-pigmented epithelial cells on the surface of the developing ciliary process.

Structure	Level	Pattern	Image	Note
TS27: ciliary body	Very strong	Regionally restricted	2B	Expression is on the non-pigmented epithelial cells on the surface of the developing ciliary process.
TS27: pigmented retinal epithelium	Strong	Regionally restricted	2B	Expression is at the basal surface of pigmented epithelium.
TS27: ciliary stroma	Present		2B

Structure	Level	Pattern	Image	Note
TS28: retina epithelium	Strong	Regionally restricted	2C	Expression is in the non-pigmented epithelium.
TS28: ciliary body	Present	Regionally restricted	2C	Expression is at the apposed basal surface of the folded pigmented epithelium of the two ciliary processes.

Structure	Level	Pattern	Image	Note
TS28: ciliary body	Strong		2D
TS28: retina epithelium	Strong	Regionally restricted	2D	Expression is in the non-pigmented epithelium.

Structure	Level	Pattern	Image	Note
TS27: retina inner plexiform layer	Present		3A
TS27: retina nerve fiber layer	Present		3A
TS27: pigmented retinal epithelium	Present	Regionally restricted	3A	Expression is at the apical and basal membrane.
TS27: neural retina	Weak	Regionally restricted	3A	Expression is in undifferentiated neuroblastic tissue.

Structure	Level	Pattern	Image	Note
TS27: pigmented retinal epithelium	Present	Regionally restricted	3B	Expression is at the apical and basal membrane.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium		Very strong	Regionally restricted	4A	Expression is at the apical and basal membrane.
TS28: retina ganglion cell layer		Present		4A
TS28: retina outer nuclear layer		Present		4A
TS28: retina inner nuclear layer	Mueller cell	Present	Graded	4A	Expression is near the optic nerve and decreases and becomes absent in undifferentiated cells anteriorly.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium		Very strong	Regionally restricted	4B	Expression is at the apical and basal membrane.
TS28: retina ganglion cell layer		Present		4B
TS28: retina outer nuclear layer		Present		4B
TS28: retina inner nuclear layer	Mueller cell	Present	Graded	4B	Expression is near the optic nerve and decreases and becomes absent in undifferentiated cells anteriorly.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina nerve fiber layer		Strong		4C
TS28: retina outer plexiform layer		Strong		4C
TS28: retina inner nuclear layer	Mueller cell	Strong	Non-Uniform	4C	Expression is stronger in the posterior half.
TS28: retina outer nuclear layer		Present		4C

Structure	Cell Type	Level	Pattern	Image	Note
TS28: photoreceptor layer inner segment		Present		4D
TS28: photoreceptor layer outer segment		Present		4D
TS28: retina nerve fiber layer		Present		4D
TS28: retina outer nuclear layer		Present		4D
TS28: retina outer plexiform layer		Present		4D
TS28: retina inner nuclear layer	Mueller cell	Present		4D

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Very strong		5A
TS28: pigmented retinal epithelium	Strong		5A
TS28: retina inner nuclear layer	Present		5A
TS28: photoreceptor layer inner segment	Moderate		5A

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Very strong	Regionally restricted	5B	Expression is in microvilli that interdigitate into the outer segment from the retinal pigmented epithelium.
TS28: pigmented retinal epithelium	Strong		5B
TS28: photoreceptor layer inner segment	Present		5B

Structure	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium	Present	Regionally restricted	6C , 6A	Expression is at the apical and basal membrane. Anterior to the ora serrata where the neural retina ends little or no labelling was seen on the apical surface after this point.
TS28: photoreceptor layer inner segment	Present		6A , 6C

Structure	Level	Pattern	Image	Note
TS26: pigmented retinal epithelium	Present			Expression is at the apical and basal surface of pigmented epithelium.