Gene Expression Data
Assay Details
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Assay
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Reference:
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J:159448
Mende Y, et al., Deficiency of the splicing factor Sfrs10 results in early embryonic lethality in mice and has no impact on full-length SMN/Smn splicing. Hum Mol Genet. 2010 Jun 1;19(11):2154-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7285994
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Gene symbol:
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Tra2b
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Gene name:
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transformer 2 beta
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Antibody:
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anti-SFRS10
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
4A +/+ or +/-:
embryonic day 6.5
(more  )
Note: Genetic background was either wild type or heterozygous.
Specimen
4A +/+ or +/-:
(close  )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * C57BL/6N
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Genetic background was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
Ubiquitous |
4A (left)
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Ubiquitous expression in nuclei of both embryonic and extraembryonic compartments.
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| TS9: extraembryonic component |
Present |
Ubiquitous |
4A (left)
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Ubiquitous expression in nuclei of both embryonic and extraembryonic compartments.
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Specimen
4A maternal (left):
postnatal adult; Tra2btm1.2Bwir/Tra2b +
(more )
Specimen
4A maternal (left):
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * C57BL/6N
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Mutant Allele(s):
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Tra2btm1.2Bwir/Tra2b+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Present |
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4A (left)
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Specimen
4A -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * C57BL/6N
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Mutant Allele(s):
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Tra2btm1.2Bwir/Tra2btm1.2Bwir
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Absent |
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4A (right)
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Specimen
4A maternal (right):
postnatal adult; Tra2btm1.2Bwir/Tra2b +
(more )
Specimen
4A maternal (right):
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * C57BL/6N
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Mutant Allele(s):
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Tra2btm1.2Bwir/Tra2b+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Present |
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4A (right)
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Specimen
4B +/+ or +/-:
embryonic day 7.5
(more )
Note: Genetic background was either wild type or heterozygous.
Specimen
4B +/+ or +/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * C57BL/6N
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Genetic background was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
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4B (left)
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Ubiquitous expression in nuclei of both embryonic and extraembryonic compartments.
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| TS11: extraembryonic component |
Present |
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4B (left)
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Ubiquitous expression in nuclei of both embryonic and extraembryonic compartments.
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Specimen
4B maternal (left):
postnatal adult; Tra2btm1.2Bwir/Tra2b +
(more )
Specimen
4B maternal (left):
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * C57BL/6N
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Mutant Allele(s):
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Tra2btm1.2Bwir/Tra2b+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Present |
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4B (left)
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Specimen
4B -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * C57BL/6N
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Mutant Allele(s):
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Tra2btm1.2Bwir/Tra2btm1.2Bwir
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Absent |
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4B (right)
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Specimen
4B maternal (right):
postnatal adult; Tra2btm1.2Bwir/Tra2b +
(more )
Specimen
4B maternal (right):
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * C57BL/6N
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Mutant Allele(s):
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Tra2btm1.2Bwir/Tra2b+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Present |
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4B (right)
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Specimen
6A Sfrs10:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * C57BL/6N * SJL
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Mutant Allele(s):
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Tra2btm1.1Bwir/Tra2btm1.1Bwir
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Sfrs10 (Tra2b); blue - Smn1 (assay MGI:7285995).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord |
motor neuron |
Present |
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6A
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Specimen
6B Sfrs10:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJae * C57BL/6 * C57BL/6N * SJL
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Mutant Allele(s):
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Mnx1tm4(cre)Tmj/Mnx1+
Tra2btm1.1Bwir/Tra2btm1.1Bwir
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. Double labeled: brown - Sfrs10 (Tra2b); blue - Smn1 (assay MGI:7285995).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord |
motor neuron |
Absent |
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6B
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| TS28: spinal cord |
glial cell |
Present |
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6B
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Expression present in other neurons and glial cells.
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| TS28: spinal cord |
neuron |
Present |
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6B
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Expression present in other neurons and glial cells.
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Analysis Tools