Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:72517
Hajihosseini MK, et al., A splicing switch and gain-of-function mutation in FgfR2-IIIc hemizygotes causes Apert/Pfeiffer-syndrome-like phenotypes. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3855-60
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7284942
|
|
Gene symbol:
|
Fgfr2
|
|
Gene name:
|
fibroblast growth factor receptor 2
|
|
Probe:
|
FgfR2-Tk probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
5B:
embryonic day 17.5
(more  )
Specimen
5B:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: coronal suture |
Present |
Regionally restricted |
5B
|
Expression was detected in the osteogenic fronts of the coronal sutures
|
| TS25: frontal bone |
Present |
Regionally restricted |
5B
|
Expression was detected in the anterior rims of the frontal bones.
|
| TS25: frontal suture |
Present |
|
5B
|
|
| TS25: nasal bone |
Present |
Regionally restricted |
5B
|
Expression was detected in the rims of the nasal bones.
|
Specimen
5C:
embryonic day 17.5
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: chondrocranium |
Present |
Regionally restricted |
5C
|
Staining was seen in the bone of the lower rim of the eye socket, the zygomatic arch, and the intervening joints.
|
| TS25: zygomatic bone |
Present |
Regionally restricted |
5C
|
Staining was seen in the bone of the lower rim of the eye socket, the zygomatic arch, and the intervening joints.
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * FVB/N
|
|
Mutant Allele(s):
|
Fgfr2tm2.3Dsn/Fgfr2+
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: chondrocranium |
Present |
Regionally restricted |
5D
|
Staining was seen in the bone of the lower rim of the eye socket, the zygomatic arch, and the intervening joints.
|
| TS25: zygomatic bone |
Present |
Regionally restricted |
5D
|
Staining was seen in the bone of the lower rim of the eye socket, the zygomatic arch, and the intervening joints.
|
Specimen
not shown:
embryonic day 17.5; Fgfr2tm2.3Dsn/Fgfr2 +
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * FVB/N
|
|
Mutant Allele(s):
|
Fgfr2tm2.3Dsn/Fgfr2+
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: coronal suture |
Present |
|
|
Staining was less intense in hemizygotes.
|
Specimen
5K:
embryonic day 18.5
(more )
Specimen
5K:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: sternebra |
Strong |
Regionally restricted |
5K
|
Strong label was detected on the periosteal region of each sternebrae.
|
| TS26: xiphisternum |
Present |
Regionally restricted |
5K
|
Expression was detected on the periphery of the xiphoid process.
|
Specimen
5L:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * FVB/N
|
|
Mutant Allele(s):
|
Fgfr2tm2.3Dsn/Fgfr2+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: sternebra |
Strong |
Regionally restricted |
5L
|
Strong label was detected on the periosteal region of each sternebrae. Expression was also detected in the hemi-sternebrae medial rims in hemizygotes.
|
| TS26: xiphisternum |
Present |
Regionally restricted |
5L
|
Expression was detected in the periphery of the xiphoid process, which was bifurcated in hemizygotes.
|
|
Analysis Tools