Gene Expression Data
Assay Details
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Assay
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Reference:
|
J:319644
Ruan J, et al., Novel Myh11 Dual Reporter Mouse Model Provides Definitive Labeling and Identification of Smooth Muscle Cells-Brief Report. Arterioscler Thromb Vasc Biol. 2021 Feb;41(2):815-821
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|
Assay type:
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In situ reporter (knock in)
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|
MGI Accession ID:
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MGI:7281118
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|
Gene symbol:
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Myh11
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|
Gene name:
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myosin, heavy polypeptide 11, smooth muscle
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|
Reporter:
|
GFP
|
|
|
Direct Detection
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Results
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Specimen
1K1:
(close  )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1K2:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: gut |
Present |
|
1K2
|
|
Specimen
1K3:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1L:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1M:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1N:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 30
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1O:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 30
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1P:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1Q1:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more  )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
1Q1:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: artery smooth muscle |
smooth muscle cell of the coronary artery |
Present |
|
1Q1
|
Co-staining with the Myh11 antibody.
|
| TS28: coronary artery |
smooth muscle cell of the coronary artery |
Present |
|
1Q1
|
Co-staining with the Myh11 antibody.
|
Specimen
1Q2:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
1Q2:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: esophagus smooth muscle |
smooth muscle cell of the esophagus |
Present |
|
1Q2
|
Co-staining with the Myh11 antibody.
|
Specimen
1Q3:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
1Q3:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: myometrium |
uterine smooth muscle cell |
Present |
|
1Q3
|
Co-staining with the Myh11 antibody.
|
Specimen
1R1:
embryonic day 9.5; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
1R1:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: dorsal aorta |
aortic smooth muscle cell |
Present |
Single cells |
1R1
|
|
Specimen
1R2:
embryonic day 10.5; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
1R2:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
aortic smooth muscle cell |
Present |
|
1R2
|
Co-staining with the Myh11 antibody.
|
Specimen
1R3:
embryonic day 11.5; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
1R3:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: dorsal aorta |
aortic smooth muscle cell |
Present |
|
1R3
|
Co-staining with the Myh11 antibody.
|
Specimen
1S:
postnatal day 45; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
1S:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 45
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
Specimen
1T:
postnatal day 45; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Flt4 (assay MGI:7281144).
Specimen
1T:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 45
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Flt4 (assay MGI:7281144).
|
Specimen
1U:
postnatal day 45; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
1U:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 45
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: intestinal villus |
smooth muscle cell of small intestine |
Present |
|
1U2
,
1U3
,
1U1
|
Co-staining with the Myh11 antibody.
|
Specimen
1V:
postnatal day 45; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Flt4 (assay MGI:7281144).
Specimen
1V:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 45
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Flt4 (assay MGI:7281144).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: intestinal villus |
Present |
Regionally restricted |
1V2
,
1V1
,
1V3
|
Co-expressed with Flt4 in the smooth muscle cells associated with lacteals.
|
Specimen
S2D:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S2E:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S2F:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S3C1:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: aorta |
Present |
|
S3C1
|
|
Specimen
S3C2:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S3C3:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: gut |
Present |
|
S3C3
|
|
Specimen
S3C4:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S3C5:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S3D1:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: aorta |
Present |
|
S3D1
|
|
Specimen
S3D2:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S3D3:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: gut |
Present |
|
S3D3
|
|
Specimen
S3D4:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S3D5:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: uterus |
Present |
|
S3D5
|
|
Specimen
S3E1:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3E1:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: aorta smooth muscle |
aortic smooth muscle cell |
Present |
|
S3E1
|
Co-staining with the Myh11 antibody.
|
Specimen
S3E2:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3E2:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: gut |
blood vessel smooth muscle cell |
Present |
|
S3E2
|
Co-staining with the Myh11 antibody.
|
| TS28: gut |
enteric smooth muscle cell |
Present |
|
S3E2
|
Co-staining with the Myh11 antibody.
|
Specimen
S3E3:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3E3:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
Specimen
S3E4:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3E4:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spleen smooth muscle |
smooth muscle cell |
Present |
|
S3E4
|
Co-staining with the Myh11 antibody.
|
Specimen
S3E5:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3E5:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
Specimen
S3F1:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3F1:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: aorta smooth muscle |
aortic smooth muscle cell |
Present |
|
S3F1
|
Co-staining with the Myh11 antibody.
|
Specimen
S3F2:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3F2:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: gut |
blood vessel smooth muscle cell |
Present |
|
S3F2
|
Co-staining with the Myh11 antibody.
|
| TS28: gut |
enteric smooth muscle cell |
Present |
|
S3F2
|
Co-staining with the Myh11 antibody.
|
Specimen
S3F3:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3F3:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
Specimen
S3F4:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3F4:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spleen smooth muscle |
smooth muscle cell |
Present |
|
S3F4
|
Co-staining with the Myh11 antibody.
|
Specimen
S3F5:
postnatal day 60; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S3F5:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: myometrium |
uterine smooth muscle cell |
Present |
|
S3F5
|
Co-staining with the Myh11 antibody.
|
Specimen
S4B1:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S4B2:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S4B3:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S4B4:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S4B5:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
S4C:
postnatal; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S4C:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: gut |
blood vessel smooth muscle cell |
Present |
|
S4C2
,
S4C1
,
S4C3
|
Co-staining with the Myh11 antibody.
|
| TS28: gut |
enteric smooth muscle cell |
Present |
|
S4C2
,
S4C1
,
S4C3
|
Co-staining with the Myh11 antibody.
|
Specimen
S4D:
postnatal; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S4D:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: artery smooth muscle |
smooth muscle cell of the coronary artery |
Present |
|
S4D1
,
S4D2
,
S4D3
|
Co-staining with the Myh11 antibody.
|
| TS28: coronary artery |
smooth muscle cell of the coronary artery |
Present |
|
S4D1
,
S4D2
,
S4D3
|
Co-staining with the Myh11 antibody.
|
Specimen
S4E:
postnatal; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S4E:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
postnatal
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spleen smooth muscle |
smooth muscle cell |
Present |
|
S4E1
,
S4E3
,
S4E2
|
Co-staining with the Myh11 antibody.
|
Specimen
S5A:
embryonic day 10.5; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
Specimen
S5A:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281142).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
aortic smooth muscle cell |
Present |
|
S5A4
,
S5A3
,
S5A2
|
Co-staining with the Myh11 antibody.
|
Specimen
S5B:
embryonic day 10.5; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281147).
Specimen
S5B:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281147).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
aortic smooth muscle cell |
Present |
|
S5B3
,
S5B4
,
S5B2
|
|
Specimen
S5C:
embryonic day 10.5; Myh11tm1.2Ccai/Myh11 +
(more )
Note: Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281148).
Specimen
S5C:
(close )
|
Genetic Background:
|
involves: 129 * 129S4/SvJae * Black Swiss
|
|
Mutant Allele(s):
|
Myh11tm1.2Ccai/Myh11+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Myh11 reporter; red - Myh11 protein (assay MGI:7281148).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
aortic smooth muscle cell |
Present |
|
S5C2
,
S5C3
,
S5C4
|
|
|
Analysis Tools