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Gene Expression Data
Assay Details
Assay
Reference: J:319644 Ruan J, et al., Novel Myh11 Dual Reporter Mouse Model Provides Definitive Labeling and Identification of Smooth Muscle Cells-Brief Report. Arterioscler Thromb Vasc Biol. 2021 Feb;41(2):815-821
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7281142
Gene symbol: Myh11
Gene name: myosin, heavy polypeptide 11, smooth muscle
Antibody: Anti-smooth muscle Myosin heavy chain 11 antibody ab53219
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1Q1: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: artery smooth muscle smooth muscle cell of the coronary artery Present 1Q1
TS28: coronary artery smooth muscle cell of the coronary artery Present 1Q1
Specimen 1Q2: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: esophagus smooth muscle smooth muscle cell of the esophagus Present 1Q2
Specimen 1Q3: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: myometrium uterine smooth muscle cell Present 1Q3
Specimen 1R1: embryonic day 9.5; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS15: dorsal aorta aortic smooth muscle cell Absent 1R1
Specimen 1R2: embryonic day 10.5; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic smooth muscle cell Present 1R2
Specimen 1R3: embryonic day 11.5; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta aortic smooth muscle cell Present 1R3
Specimen 1S: postnatal day 45; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: artery smooth muscle blood vessel smooth muscle cell Present 1S1
TS28: mesenteric artery blood vessel smooth muscle cell Present 1S1
TS28: lymphatic vessel smooth muscle lymphatic vessel smooth muscle cell Present 1S3 , 1S1 , 1S2
Specimen 1U: postnatal day 45; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: intestinal villus smooth muscle cell of small intestine Present 1U3 , 1U2 , 1U1
Specimen S3E1: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: aorta smooth muscle aortic smooth muscle cell Present S3E1
Specimen S3E2: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: gut blood vessel smooth muscle cell Present S3E2
TS28: gut enteric smooth muscle cell Present S3E2
Specimen S3E3: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: respiratory system smooth muscle tracheobronchial smooth muscle cell Present S3E3
Specimen S3E4: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: spleen smooth muscle smooth muscle cell Present S3E4
Specimen S3E5: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: muscle of ductus deferens smooth muscle cell Present S3E5
Specimen S3F1: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: aorta smooth muscle aortic smooth muscle cell Present S3F1
Specimen S3F2: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: gut blood vessel smooth muscle cell Present S3F2
TS28: gut enteric smooth muscle cell Present S3F2
Specimen S3F3: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: respiratory system smooth muscle tracheobronchial smooth muscle cell Present S3F3
Specimen S3F4: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: spleen smooth muscle smooth muscle cell Present S3F4
Specimen S3F5: postnatal day 60; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: myometrium uterine smooth muscle cell Present S3F5
Specimen S4C: postnatal; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: gut blood vessel smooth muscle cell Present S4C2 , S4C3
TS28: gut enteric smooth muscle cell Present S4C2 , S4C3
Specimen S4D: postnatal; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: artery smooth muscle smooth muscle cell of the coronary artery Present S4D3 , S4D2
TS28: coronary artery smooth muscle cell of the coronary artery Present S4D3 , S4D2
Specimen S4E: postnatal; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: spleen smooth muscle smooth muscle cell Present S4E3 , S4E2
Specimen S5A: embryonic day 10.5; Myh11tm1.2Ccai/Myh11+ (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta aortic smooth muscle cell Present S5A3 , S5A4 , S5A1
Specimen S5D: postnatal day 60 (more )
Note: Double labeled: red - Myh11 protein; green - Myh11 reporter (assay MGI:7281118).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel smooth muscle smooth muscle cell of the coronary artery Present S5D2 , S5D3 , S5D1 , S5D4
TS28: coronary artery smooth muscle cell of the coronary artery Present S5D2 , S5D3 , S5D1 , S5D4
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory