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Gene Expression Data
Assay Details
Assay
Reference: J:312489 Tang W, et al., Hlf Expression Marks Early Emergence of Hematopoietic Stem Cell Precursors With Adult Repopulating Potential and Fate. Front Cell Dev Biol. 2021;9:728057
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7263011
Gene symbol: Runx1
Gene name: runt related transcription factor 1
Antibody: anti-RUNX1/AML1 + RUNX2 + RUNX3 (EPR3099; ab92336)
Detection system: Secondary antibody
Results
Specimen 1E: embryonic day 10.5; Hlfem1Bcgen/Hlf+ (more )
Note: Triple labeled: blue - Runx1; red - Pecam1 (assay MGI:7263009); green - Hlf reporter (assay MGI:7263028).
Structure Level Pattern Image Note
TS17: aorta-gonad-mesonephros Present Regionally restricted 1E left , 1E center Expression was detected in intraaortic hematopoietic clusters (IAHCs) in the AGM. Co-expression was noted with Pecam1 and Hlf-tdTomato.
Specimen 4B E9.5: embryonic day 9.5; Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Triple labeled: blue - Runx1; red - Pecam1 (assay MGI:7263009); green - Hlf reporter (assay MGI:7262859).
Structure Level Pattern Image Note
TS15: dorsal aorta Present 4B E9.5 center , 4B E9.5 left
TS15: hematopoietic system Present 4B E9.5 center , 4B E9.5 left
Specimen 4B E10.0: embryonic day 10.0; Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Triple labeled: blue - Runx1; red - Pecam1 (assay MGI:7263009); green - Hlf reporter (assay MGI:7262859).
Structure Level Pattern Image Note
TS16: dorsal aorta Present 4B E10.0 center , 4B E10.0 left
TS16: hematopoietic system Present 4B E10.0 center , 4B E10.0 left
Specimen 4B E10.5: embryonic day 10.5; Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Triple labeled: blue - Runx1; red - Pecam1 (assay MGI:7263009); green - Hlf reporter (assay MGI:7262859).
Structure Level Pattern Image Note
TS17: dorsal aorta Present 4B E10.5 left , 4B E10.5 center
TS17: hematopoietic system Present 4B E10.5 left , 4B E10.5 center
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory