About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:312489 Tang W, et al., Hlf Expression Marks Early Emergence of Hematopoietic Stem Cell Precursors With Adult Repopulating Potential and Fate. Front Cell Dev Biol. 2021;9:728057
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7262859
Gene symbol: Hlf
Gene name: hepatic leukemia factor
Reporter: ESR
Antibody: anti-ERalpha (SP1; ab16660)
Detection system: Secondary antibody
Results
Specimen 4B E9.5: embryonic day 9.5; Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Triple labeled: green - Hlf reporter; blue - Runx1 (assay MGI:7263011); red - Pecam1 (assay MGI:7263009).
Structure Cell Type Level Pattern Image Note
TS15: aorta-gonad-mesonephros hematopoietic precursor cell Present Single cells 4B E9.5 right , 4B E9.5 left , 4B E9.5 center Expression is seen in intra-aortic haematopoietic clusters (IAHCs).
TS15: dorsal aorta hematopoietic precursor cell Present Single cells 4B E9.5 right , 4B E9.5 left , 4B E9.5 center Expression is seen in intra-aortic haematopoietic clusters (IAHCs).
TS15: hematopoietic system hematopoietic precursor cell Present Single cells 4B E9.5 right , 4B E9.5 left , 4B E9.5 center Expression is seen in intra-aortic haematopoietic clusters (IAHCs).

Specimen 4B E10.0: embryonic day 10.0; Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Triple labeled: green - Hlf reporter; blue - Runx1 (assay MGI:7263011); red - Pecam1 (assay MGI:7263009).
Structure Cell Type Level Pattern Image Note
TS16: aorta-gonad-mesonephros hematopoietic precursor cell Present Single cells 4B E10.0 left , 4B E10.0 right , 4B E10.0 center Expression is seen in intra-aortic haematopoietic clusters (IAHCs).
TS16: dorsal aorta hematopoietic precursor cell Present Single cells 4B E10.0 left , 4B E10.0 right , 4B E10.0 center Expression is seen in intra-aortic haematopoietic clusters (IAHCs).
TS16: hematopoietic system hematopoietic precursor cell Present Single cells 4B E10.0 left , 4B E10.0 right , 4B E10.0 center Expression is seen in intra-aortic haematopoietic clusters (IAHCs).

Specimen 4B E10.5: embryonic day 10.5; Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Triple labeled: green - Hlf reporter; blue - Runx1 (assay MGI:7263011); red - Pecam1 (assay MGI:7263009).
Structure Cell Type Level Pattern Image Note
TS17: aorta-gonad-mesonephros hematopoietic precursor cell Present Single cells 4B E10.5 left , 4B E10.5 right , 4B E10.5 center Expression is seen in intra-aortic haematopoietic clusters (IAHCs).
TS17: dorsal aorta hematopoietic precursor cell Present Single cells 4B E10.5 left , 4B E10.5 right , 4B E10.5 center Expression is seen in intra-aortic haematopoietic clusters (IAHCs).
TS17: hematopoietic system hematopoietic precursor cell Present Single cells 4B E10.5 left , 4B E10.5 right , 4B E10.5 center Expression is seen in intra-aortic haematopoietic clusters (IAHCs).


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
05/21/2024
MGI 6.23
The Jackson Laboratory