Antibody:	Mouse/Rat Neuropilin-2 Antibody (AF567)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS21: back skin	Present	Regionally restricted	2A	Expressed in the dermal lymphatic sprouts.
TS21: dermis	Present	Regionally restricted	2A	Expressed in the dermal lymphatic sprouts.
TS21: lymph sac	Present	Regionally restricted	2A	Expressed in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS21: back skin	Present	Regionally restricted	2B	Expressed in the dermal lymphatic sprouts, which were dilated and failed to migrate toward the midline of the embryo. There was an increased number of cells in lymphatic vessels.
TS21: dermis	Present	Regionally restricted	2B	Expressed in the dermal lymphatic sprouts, which were dilated and failed to migrate toward the midline of the embryo. There was an increased number of cells in lymphatic vessels.
TS21: lymph sac	Present	Regionally restricted	2B	Expressed in the dermal lymphatic sprouts, which were dilated and failed to migrate toward the midline of the embryo. There was an increased number of cells in lymphatic vessels.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3A	Expressed in the dermal lymphatic sprouts.
TS22: dermis	Present	Regionally restricted	3A	Expressed in the dermal lymphatic sprouts.
TS22: lymphatic vessel	Present	Regionally restricted	3A	Expressed in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3B	Expressed in the dermal lymphatic sprouts. Mutant dermal lymphatic network had a marked reduction in branching, increased vessel width and increased numbers of cells per vessel.
TS22: dermis	Present	Regionally restricted	3B	Expressed in the dermal lymphatic sprouts. Mutant dermal lymphatic network had a marked reduction in branching, increased vessel width and increased numbers of cells per vessel.
TS22: lymphatic vessel	Present	Regionally restricted	3B	Expressed in the dermal lymphatic sprouts. Mutant dermal lymphatic network had a marked reduction in branching, increased vessel width and increased numbers of cells per vessel.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3C	Expressed in the dermal lymphatic sprouts.
TS22: dermis	Present	Regionally restricted	3C	Expressed in the dermal lymphatic sprouts.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	3C	Expressed in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3D	Expressed in the dermal lymphatic sprouts. The mutant sprouts displayed a stereotypical blunt-tipped and widened morphology.
TS22: dermis	Present	Regionally restricted	3D	Expressed in the dermal lymphatic sprouts. The mutant sprouts displayed a stereotypical blunt-tipped and widened morphology.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	3D	Expressed in the dermal lymphatic sprouts. The mutant sprouts displayed a stereotypical blunt-tipped and widened morphology.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4A	Expressed in the dermal lymphatic sprouts.
TS22: dermis	Present	Regionally restricted	4A	Expressed in the dermal lymphatic sprouts.
TS22: lymphatic vessel	Present	Regionally restricted	4A	Expressed in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4B	Expressed in the dermal lymphatic sprouts.
TS22: dermis	Present	Regionally restricted	4B	Expressed in the dermal lymphatic sprouts.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4B	Expressed in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4C	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: dermis	Present	Regionally restricted	4C	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: lymphatic vessel	Present	Regionally restricted	4C	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4D	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: dermis	Present	Regionally restricted	4D	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4D	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4E	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: dermis	Present	Regionally restricted	4E	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: lymphatic vessel	Present	Regionally restricted	4E	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4F	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: dermis	Present	Regionally restricted	4F	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4F	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4G	Expressed in the dermal lymphatic sprouts. Dermal lymphatics were significantly thinner than in Pkd1-/- or Wnt5a-/- mutant mice.
TS22: dermis	Present	Regionally restricted	4G	Expressed in the dermal lymphatic sprouts. Dermal lymphatics were significantly thinner than in Pkd1-/- or Wnt5a-/- mutant mice.
TS22: lymphatic vessel	Present	Regionally restricted	4G	Expressed in the dermal lymphatic sprouts. Dermal lymphatics were significantly thinner than in Pkd1-/- or Wnt5a-/- mutant mice.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4H	Expressed in the dermal lymphatic sprouts. Dermal lymphatics were significantly thinner than in Pkd1-/- or Wnt5a-/- mutant mice.
TS22: dermis	Present	Regionally restricted	4H	Expressed in the dermal lymphatic sprouts. Dermal lymphatics were significantly thinner than in Pkd1-/- or Wnt5a-/- mutant mice.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4H	Expressed in the dermal lymphatic sprouts. Dermal lymphatics were significantly thinner than in Pkd1-/- or Wnt5a-/- mutant mice.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4I	Expressed in the dermal lymphatic sprouts, suppressing the widened vessel phenotype and restoring a more wild type morphology to the network overall.
TS22: dermis	Present	Regionally restricted	4I	Expressed in the dermal lymphatic sprouts, suppressing the widened vessel phenotype and restoring a more wild type morphology to the network overall.
TS22: lymphatic vessel	Present	Regionally restricted	4I	Expressed in the dermal lymphatic sprouts, suppressing the widened vessel phenotype and restoring a more wild type morphology to the network overall.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4J	Expressed in the dermal lymphatic sprouts, suppressing the widened vessel phenotype and restoring a more wild type morphology to the network overall.
TS22: dermis	Present	Regionally restricted	4J	Expressed in the dermal lymphatic sprouts, suppressing the widened vessel phenotype and restoring a more wild type morphology to the network overall.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4J	Expressed in the dermal lymphatic sprouts, suppressing the widened vessel phenotype and restoring a more wild type morphology to the network overall.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4K	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels, similar as in Wnt5a-/-.
TS22: dermis	Present	Regionally restricted	4K	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels, similar as in Wnt5a-/-.
TS22: lymphatic vessel	Present	Regionally restricted	4K	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels, similar as in Wnt5a-/-.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4L	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels, similar as in Wnt5a-/-.
TS22: dermis	Present	Regionally restricted	4L	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels, similar as in Wnt5a-/-.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4L	Expressed in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels, similar as in Wnt5a-/-.