Emcn Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:320108 Chau TCY, et al., Pkd1 and Wnt5a genetically interact to control lymphatic vascular morphogenesis in mice. Dev Dyn. 2022 Feb;251(2):336-349
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7262863
Gene symbol:	Emcn
Gene name:	endomucin

Antibody:	anti-Endomucin (sc-53941)
Detection system:	Secondary antibody coupled to Alexa Fluor 647

Results

2A ©

2A' ©

Specimen 2A/A': embryonic day 13.5 (more

)
Note: Double labeled: magenta - Emcn; gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS21: back skin	Present	Regionally restricted	2A' , 2A	Expressed in subcutaneous blood vessels.
TS21: dermis	Present	Regionally restricted	2A' , 2A	Expressed in subcutaneous blood vessels.
TS21: trunk blood vessel	Present	Regionally restricted	2A' , 2A	Expressed in subcutaneous blood vessels.

2B ©

2B' ©

Specimen 2B/B': embryonic day 13.5; Wnt5a^tm1Amc/Wnt5a^tm1Amc (more

)
Note: Double labeled: magenta - Emcn; gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS21: back skin	Present	Regionally restricted	2B , 2B'	Expressed in subcutaneous blood vessels. The blood vessel network displayed striking, abnormal endothelial cell clusters along the midline.
TS21: dermis	Present	Regionally restricted	2B , 2B'	Expressed in subcutaneous blood vessels. The blood vessel network displayed striking, abnormal endothelial cell clusters along the midline.
TS21: trunk blood vessel	Present	Regionally restricted	2B , 2B'	Expressed in subcutaneous blood vessels. The blood vessel network displayed striking, abnormal endothelial cell clusters along the midline.

3A ©

3A' ©

Specimen 3A/A': embryonic day 14.5 (more

)
Note: Triple labeled: magenta - Emcn; green - Prox1 (assay MGI:7262864); gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3A' , 3A	Expressed in subcutaneous blood vessels.
TS22: dermis	Present	Regionally restricted	3A' , 3A	Expressed in subcutaneous blood vessels.
TS22: trunk blood vessel	Present	Regionally restricted	3A' , 3A	Expressed in subcutaneous blood vessels.

3B ©

3B' ©

Specimen 3B/B': embryonic day 14.5; Ryk^tm1Stac/Ryk^tm1Stac (more

)
Note: Triple labeled: magenta - Emcn; green - Prox1 (assay MGI:7262864); gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3B , 3B'	Expressed in subcutaneous blood vessels, similar as in the wild-type.
TS22: dermis	Present	Regionally restricted	3B , 3B'	Expressed in subcutaneous blood vessels, similar as in the wild-type.
TS22: trunk blood vessel	Present	Regionally restricted	3B , 3B'	Expressed in subcutaneous blood vessels, similar as in the wild-type.

4A ©

4A' ©

Specimen 4A/A': embryonic day 14.5 (more

)
Note: Double labeled: magenta - Emcn; gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4A , 4A'	Expressed in subcutaneous blood vessels.
TS22: dermis	Present	Regionally restricted	4A , 4A'	Expressed in subcutaneous blood vessels.
TS22: trunk blood vessel	Present	Regionally restricted	4A , 4A'	Expressed in subcutaneous blood vessels.

4C ©

4C' ©

Specimen 4C/C': embryonic day 14.5; Pkd1^tm2.1Ggg/Pkd1^tm2.1Ggg (more

)
Note: Double labeled: magenta - Emcn; gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4C , 4C'	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points.
TS22: dermis	Present	Regionally restricted	4C , 4C'	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points.
TS22: trunk blood vessel	Present	Regionally restricted	4C , 4C'	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points.

4E ©

4E' ©

Specimen 4E/E': embryonic day 14.5; Wnt5a^tm1Amc/Wnt5a^tm1Amc (more

)
Note: Double labeled: magenta - Emcn; gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4E , 4E'	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points.
TS22: dermis	Present	Regionally restricted	4E , 4E'	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points.
TS22: trunk blood vessel	Present	Regionally restricted	4E , 4E'	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points.

4G ©

4G' ©

Specimen 4G/G': embryonic day 14.5; Pkd1^tm2.1Ggg/Pkd1^tm2.1Ggg, Wnt5a^tm1Amc/Wnt5a^tm1Amc (more

)
Note: Double labeled: magenta - Emcn; gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4G' , 4G	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points, similar as in Wnt5a-/- or Pkd1-/-.
TS22: dermis	Present	Regionally restricted	4G' , 4G	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points, similar as in Wnt5a-/- or Pkd1-/-.
TS22: trunk blood vessel	Present	Regionally restricted	4G' , 4G	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points, similar as in Wnt5a-/- or Pkd1-/-.

4I ©

4I' ©

Specimen 4I/I': embryonic day 14.5; Pkd1^tm2.1Ggg/Pkd1^tm2.1Ggg, Wnt5a^tm1Amc/Wnt5a⁺ (more

)
Note: Double labeled: magenta - Emcn; gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4I' , 4I	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points, similar as in Pkd1-/-.
TS22: dermis	Present	Regionally restricted	4I' , 4I	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points, similar as in Pkd1-/-.
TS22: trunk blood vessel	Present	Regionally restricted	4I' , 4I	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points, similar as in Pkd1-/-.

4K ©

4K' ©

Specimen 4K/K': embryonic day 14.5; Pkd1^tm2.1Ggg/Pkd1⁺, Wnt5a^tm1Amc/Wnt5a^tm1Amc (more

)
Note: Double labeled: magenta - Emcn; gray - Nrp2 (assay MGI:7262861).

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4K' , 4K	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points, similar as in Wnt5a-/-.
TS22: dermis	Present	Regionally restricted	4K' , 4K	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points, similar as in Wnt5a-/-.
TS22: trunk blood vessel	Present	Regionally restricted	4K' , 4K	Expressed in subcutaneous blood vessels. The blood vessel network had a reduced number of branch points, similar as in Wnt5a-/-.

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