Antibody:	anti-Prox1 (#11-002)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS21: back skin	Present	Regionally restricted	2G , 2G'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS21: dermis	Present	Regionally restricted	2G , 2G'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS21: lymph sac	Present	Regionally restricted	2G , 2G'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS21: back skin	Present	Regionally restricted	2H , 2H'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS21: dermis	Present	Regionally restricted	2H , 2H'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS21: lymph sac	Present	Regionally restricted	2H , 2H'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3A	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS22: dermis	Present	Regionally restricted	3A	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	3A	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3B	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. The mutant sprouts displayed a stereotypical blunt-tipped and widened morphology.
TS22: dermis	Present	Regionally restricted	3B	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. The mutant sprouts displayed a stereotypical blunt-tipped and widened morphology.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	3B	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. The mutant sprouts displayed a stereotypical blunt-tipped and widened morphology.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3C	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS22: dermis	Present	Regionally restricted	3C	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	3C	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	3D	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. The mutant sprouts displayed a stereotypical blunt-tipped and widened morphology.
TS22: dermis	Present	Regionally restricted	3D	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. The mutant sprouts displayed a stereotypical blunt-tipped and widened morphology.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	3D	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. The mutant sprouts displayed a stereotypical blunt-tipped and widened morphology.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4B , 4B'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS22: dermis	Present	Regionally restricted	4B , 4B'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4B , 4B'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4D' , 4D	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: dermis	Present	Regionally restricted	4D' , 4D	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4D' , 4D	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4F' , 4F	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: dermis	Present	Regionally restricted	4F' , 4F	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4F' , 4F	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4H , 4H'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Dermal lymphatics were significantly thinner than in Pkd1-/- or Wnt5a-/- mutant mice.
TS22: dermis	Present	Regionally restricted	4H , 4H'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Dermal lymphatics were significantly thinner than in Pkd1-/- or Wnt5a-/- mutant mice.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4H , 4H'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Dermal lymphatics were significantly thinner than in Pkd1-/- or Wnt5a-/- mutant mice.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4J , 4J'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts, suppressing the widened vessel phenotype and restoring a more wild type morphology to the network overall.
TS22: dermis	Present	Regionally restricted	4J , 4J'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts, suppressing the widened vessel phenotype and restoring a more wild type morphology to the network overall.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4J , 4J'	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts, suppressing the widened vessel phenotype and restoring a more wild type morphology to the network overall.

Structure	Level	Pattern	Image	Note
TS22: back skin	Present	Regionally restricted	4L' , 4L	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels, similar as in Wnt5a-/-.
TS22: dermis	Present	Regionally restricted	4L' , 4L	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels, similar as in Wnt5a-/-.
TS22: lymphatic vessel endothelium	Present	Regionally restricted	4L' , 4L	Expressed in lymphatic endothelial cells in the dermal lymphatic sprouts. Mutant lymphatic networks had significantly wider lymphatic vessels, similar as in Wnt5a-/-.