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Gene Expression Data
Assay Details
Assay
Reference: J:316478 Grzenda A, et al., Timing and expression of the angiopoietin-1-Tie-2 pathway in murine lung development and congenital diaphragmatic hernia. Dis Model Mech. 2013 Jan;6(1):106-14
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7256465
Gene symbol: Angpt1
Gene name: angiopoietin 1
Antibody: Anti-Ang-1
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 2C E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Angpt1; green - Acta2 (assay MGI:7256466).
Structure Level Pattern Image Note
TS20: lung epithelium Present Regionally restricted 2C E12.5 Ang-1 + alpha-SMA (20x) Expression was detected in the epithelium of the distal lung bud.
TS20: bronchus Absent 2C E12.5 Ang-1 + alpha-SMA (20x) Angpt1 was completely absent from smooth muscle-lined airways and vasculature.
TS20: lung vascular element Absent 2C E12.5 Ang-1 + alpha-SMA (20x) Angpt1 was completely absent from smooth muscle-lined airways and vasculature.
TS20: smooth muscle tissue Absent 2C E12.5 Ang-1 + alpha-SMA (20x) Angpt1 was completely absent from smooth muscle-lined airways and vasculature.
Specimen 2C E15.5: embryonic day 15.5 (more )
Note: Double labeled: red - Angpt1; green - Acta2 (assay MGI:7256466).
Structure Level Pattern Image Note
TS23: lung epithelium Present Regionally restricted 2C E15.5 Ang-1 + alpha-SMA (20x) Expression was detected in the epithelium of the distal lung bud.
TS23: bronchus Absent 2C E15.5 Ang-1 + alpha-SMA (20x) Angpt1 was completely absent from smooth muscle-lined airways and vasculature.
TS23: lung vascular element Absent 2C E15.5 Ang-1 + alpha-SMA (20x) Angpt1 was completely absent from smooth muscle-lined airways and vasculature.
TS23: smooth muscle tissue Absent 2C E15.5 Ang-1 + alpha-SMA (20x) Angpt1 was completely absent from smooth muscle-lined airways and vasculature.
Specimen 2C E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Angpt1; green - Acta2 (assay MGI:7256466).
Structure Level Pattern Image Note
TS26: bronchus Present Regionally restricted 2C E18.5 Ang-1 + alpha-SMA (20x) Expression shifted to the primary, central airways.
Specimen 2C PN1: postnatal day 1 (more )
Note: Double labeled: red - Angpt1; green - Acta2 (assay MGI:7256466).
Structure Level Pattern Image Note
TS27: bronchus Present Regionally restricted 2C PN1 Ang-1 + alpha-SMA (20x) Expression detected in the primary, central airways.
Specimen 3A E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Angpt1; green - Sftpc (assay MGI:7256469).
Structure Cell Type Level Pattern Image Note
TS20: lung epithelium type II pneumocyte Present Regionally restricted 3A E12.5 Ang-1 + Pro-C (40x) Expression was detected in the epithelium of the distal lung bud, where it co-localized with Sftpc in type II alveolar cells.
Specimen 3A E15.5: embryonic day 15.5 (more )
Note: Double labeled: red - Angpt1; green - Sftpc (assay MGI:7256469).
Structure Cell Type Level Pattern Image Note
TS23: lung epithelium type II pneumocyte Present Regionally restricted 3A E15.5 Ang-1 + Pro-C (40x) Expression was detected in the epithelium of the distal lung bud, where it co-localized with Sftpc in type II alveolar cells.
Specimen 3A E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Angpt1; green - Sftpc (assay MGI:7256469).
Structure Cell Type Level Pattern Image Note
TS26: lung epithelium type II pneumocyte Present Regionally restricted 3A E18.5 Ang-1 + Pro-C (40x) Expression was detected in the epithelium of the distal lung bud, where it co-localized with Sftpc in type II alveolar cells.
Specimen 3A PN1: postnatal day 1 (more )
Note: Double labeled: red - Angpt1; green - Sftpc (assay MGI:7256469).
Structure Cell Type Level Pattern Image Note
TS27: lung epithelium type II pneumocyte Present Regionally restricted 3A PN1 Ang-1 + Pro-C (40x) Expression was detected in the epithelium of the distal lung bud, where it co-localized with Sftpc in type II alveolar cells.
Specimen 3B E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Angpt1; green - Cd34 (assay MGI:7256472).
Structure Level Pattern Image Note
TS20: lung epithelium Present 3B E12.5 Ang-1 + CD34 (20x)
Specimen 3B E15.5: embryonic day 15.5 (more )
Note: Double labeled: red - Angpt1; green - Cd34 (assay MGI:7256472).
Structure Level Pattern Image Note
TS23: lung epithelium Present 3B E15.5 Ang-1 + CD34 (20x)
Specimen 3B E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Angpt1; green - Cd34 (assay MGI:7256472).
Structure Level Pattern Image Note
TS26: lung epithelium Present 3B E18.5 Ang-1 + CD34 (20x)
Specimen 3B PN1: postnatal day 1 (more )
Note: Double labeled: red - Angpt1; green - Cd34 (assay MGI:7256472).
Structure Level Pattern Image Note
TS27: lung epithelium Present 3B PN1 Ang-1 + CD34 (20x)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory