Gene Expression Data
Assay Details
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Assay
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Reference:
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J:316478
Grzenda A, et al., Timing and expression of the angiopoietin-1-Tie-2 pathway in murine lung development and congenital diaphragmatic hernia. Dis Model Mech. 2013 Jan;6(1):106-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7256469
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Gene symbol:
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Sftpc
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Gene name:
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surfactant associated protein C
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Antibody:
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Anti-pro-SPC
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3A E12.5:
embryonic day 12.5
(more  )
Note: Double labeled: green - Sftpc; red - Angpt1 (assay MGI:7256465).
Specimen
3A E12.5:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Sftpc; red - Angpt1 (assay MGI:7256465).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: lung epithelium |
type II pneumocyte |
Present |
Regionally restricted |
3A E12.5 Ang-1 + Pro-C (40x)
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Expression was detected in type II alveloar cells in the distal lung epithelium, where it co-localized with Angpt1.
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Specimen
3A E15.5:
embryonic day 15.5
(more )
Note: Double labeled: green - Sftpc; red - Angpt1 (assay MGI:7256465).
Specimen
3A E15.5:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Sftpc; red - Angpt1 (assay MGI:7256465).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: lung epithelium |
type II pneumocyte |
Present |
Regionally restricted |
3A E15.5 Ang-1 + Pro-C (40x)
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Expression was detected in type II alveloar cells in the distal lung epithelium, where it co-localized with Angpt1.
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Specimen
3A E18.5:
embryonic day 18.5
(more )
Note: Double labeled: green - Sftpc; red - Angpt1 (assay MGI:7256465).
Specimen
3A E18.5:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Sftpc; red - Angpt1 (assay MGI:7256465).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
type II pneumocyte |
Present |
Regionally restricted |
3A E18.5 Ang-1 + Pro-C (40x)
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Expression was detected in type II alveloar cells in the distal lung epithelium, where it co-localized with Angpt1.
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Specimen
3A PN1:
postnatal day 1
(more )
Note: Double labeled: green - Sftpc; red - Angpt1 (assay MGI:7256465).
Specimen
3A PN1:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Sftpc; red - Angpt1 (assay MGI:7256465).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lung epithelium |
type II pneumocyte |
Present |
Regionally restricted |
3A PN1 Ang-1 + Pro-C (40x)
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Expression was detected in type II alveloar cells in the distal lung epithelium, where it co-localized with Angpt1.
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Analysis Tools