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Gene Expression Data
Assay Details
Assay
Reference: J:315320 Moore R, et al., The primitive endoderm segregates from the epiblast in beta1 integrin-deficient early mouse embryos. Mol Cell Biol. 2014 Feb;34(3):560-72
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6871024
Gene symbol: Dab2
Gene name: disabled 2, mitogen-responsive phosphoprotein
Antibody: anti-Disabled-2
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-Dab2 MGI:6871023 may have been used in this assay.
Results
Specimen 1A E4.5 WT: embryonic day 4.5 (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 1A E4.5 WT Expression in primitive endoderm forming a monolayer epithelium covering inner cell mass.
Specimen 1A E4.5 Intb1 (-/-): embryonic day 4.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS6: primitive endoderm Present Regionally restricted 1A E4.5 Intb1 (-/-) Expression in primitive endoderm forming multiple layers next to cluster of epiblast cells.
Specimen 1A 5.5 WT: embryonic day 5.5 (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 1A E5.5 WT
Specimen 1A E5.5 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present Clusters 1A E5.5 Intb1 (-/-)
Specimen 1B section #1 WT: embryonic day 5.5 (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 1B section #1 WT
Specimen 1B section #1 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present Clusters 1B section #1 Intb1 (-/-) Expression in endoderm cells segregating away from epiblast cells.
Specimen 1B section #2 WT: embryonic day 5.5 (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 1B section #2 WT
Specimen 1B section #2 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present Clusters 1B section #2 Intb1 (-/-) Expression in endoderm cells segregating away from epiblast cells.
Specimen 1B section #3 WT: embryonic day 5.5 (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 1B section #3 WT
Specimen 1B section #3 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present Clusters 1B section #3 Intb1 (-/-) Expression in endoderm cells segregating away from epiblast cells.
Specimen 1B section #4 WT: embryonic day 5.5 (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 1B section #4 WT
Specimen 1B section #4 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present Clusters 1B section #4 Intb1 (-/-) Expression in endoderm cells segregating away from epiblast cells.
Specimen 1B section #5 WT: embryonic day 5.5 (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 1B section #5 WT
Specimen 1B section #5 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present Clusters 1B section #5 Intb1 (-/-) Expression in endoderm cells segregating away from epiblast cells.
Specimen 1B section #6 WT: embryonic day 5.5 (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 1B section #6 WT
Specimen 1B section $6 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present Clusters 1B section #6 Intb1 (-/-) Expression in endoderm cells segregating away from epiblast cells.
Specimen 1C WT: embryonic day 5.5 (more )
Note: Double labeled: green - Dab2; red - laminin.
Structure Level Pattern Image Note
TS7: primitive endoderm Present Regionally restricted 1C WT (right) , 1C WT (middle) Expression in concentric monolayer of endoderm enveloping the central epiblast.
Specimen 1C Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Dab2; red - laminin.
Structure Level Pattern Image Note
TS7: primitive endoderm Present Patchy 1C Intb1 (-/-) (middle) , 1C Intb1 (-/-) (right) Expression in patch of cells, some of which are also positive for laminin expression. No epithelial organization in recognizable.
Specimen 6A (left): embryonic day 5.5; Itgb1tm1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Dab2; red - laminin.
Structure Level Pattern Image Note
TS7: primitive endoderm Present 6A (left)
Specimen 6A (right): embryonic day 5.5; Itgb1tm1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 6A (right)
Specimen 6B (left): embryonic day 5.5; Itgb1tm1Efu/Itgb1tm1.1Efu, Tg(Ttr-cre)1Hadj/0 (more )
Note: Conditional mutant. Double labeled: green - Dab2; red - laminin.
Structure Level Pattern Image Note
TS7: primitive endoderm Present Regionally restricted 6B (left) Expression in mislocalized endoderm cell in interior of embryo.
Specimen 6C (left): embryonic day 5.5; Edil3Tg(Sox2-cre)1Amc/0, Itgb1tm1Efu/Itgb1tm1.1Efu (more )
Note: Conditional mutant. Double labeled: green - Dab2; red - laminin.
Structure Level Pattern Image Note
TS7: primitive endoderm Present Clusters 6C (left)
Specimen 6C (right): embryonic day 5.5; Edil3Tg(Sox2-cre)1Amc/0, Itgb1tm1Efu/Itgb1tm1.1Efu (more )
Note: Conditional mutant. Double labeled: red - Dab2; green - Pou5f1 (assay MGI:6871026).
Structure Level Pattern Image Note
TS7: primitive endoderm Present Clusters 6C (right)
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory