About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:315320 Moore R, et al., The primitive endoderm segregates from the epiblast in beta1 integrin-deficient early mouse embryos. Mol Cell Biol. 2014 Feb;34(3):560-72
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6871026
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct3/4 (C-10; sc-5279)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A E4.5 WT: embryonic day 4.5 (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS6: epiblast Present 1A E4.5 WT Expression in inner cell mass.
Specimen 1A E4.5 Intb1 (-/-): embryonic day 4.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS6: epiblast Present Clusters 1A E4.5 Intb1 (-/-) Expression in cluster of epiblast cells.
Specimen 1A 5.5 WT: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 1A E5.5 WT
Specimen 1A E5.5 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present Clusters 1A E5.5 Intb1 (-/-) Expression in cluster of epiblast cells.
Specimen 1B section #1 WT: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 1B section #1 WT
Specimen 1B section #1 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 1B section #1 Intb1 (-/-)
Specimen 1B section #2 WT: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 1B section #2 WT
Specimen 1B section #2 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 1B section #2 Intb1 (-/-)
Specimen 1B section #3 WT: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 1B section #3 WT
Specimen 1B section #3 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 1B section #3 Intb1 (-/-)
Specimen 1B section #4 WT: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 1B section #4 WT
Specimen 1B section #4 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Ambiguous Regionally restricted 1B section #4 Intb1 (-/-)
Specimen 1B section #5 WT: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 1B section #5 WT
Specimen 1B section #5 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Ambiguous Regionally restricted 1B section #5 Intb1 (-/-)
Specimen 1B section #6 WT: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Ambiguous Regionally restricted 1B section #6 WT
Specimen 1B section $6 Intb1 (-/-): embryonic day 5.5; Itgb1tm1.1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Ambiguous Regionally restricted 1B section #6 Intb1 (-/-)
Specimen 6A (right): embryonic day 5.5; Itgb1tm1Efu/Itgb1tm1.1Efu (more )
Note: Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 6A (right)
Specimen 6C (right): embryonic day 5.5; Edil3Tg(Sox2-cre)1Amc/0, Itgb1tm1Efu/Itgb1tm1.1Efu (more )
Note: Conditional mutant. Double labeled: green - Pou5f1; red - Dab2 (assay MGI:6871024).
Structure Level Pattern Image Note
TS7: epiblast Present 6C (right)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory