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Gene Expression Data
Assay Details
Assay
Reference: J:308824 de Guia RM, et al., Ablation of Nampt in AgRP neurons leads to neurodegeneration and impairs fasting- and ghrelin-mediated food intake. FASEB J. 2021 May;35(5):e21450
Assay type: RT-PCR
MGI Accession ID: MGI:6850147
Gene symbol: Lepr
Gene name: leptin receptor
Probe: Lepr-pC, Lepr-pD
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR was used to obtain these results. Gene expression was normalized to either 18S rRNA content or to total cDNA content.
Results Image: 4H
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
E12 WT E12.5 (a) TS20: head Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteNot Specified
E12 ARNKO E12.5 (a) TS20: head Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Not Specified Conditional mutant.
E16 WT E16.5 (a) TS24: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteNot Specified
E16 ARNKO E16.5 (a) TS24: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Not Specified Conditional mutant.
P0 WT P0 TS27: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteNot Specified
P0 ARNKO P0 TS27: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Not Specified Conditional mutant.
P10 WT P10 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteNot Specified
P10 ARNKO P10 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Not Specified Conditional mutant.
P21 WT P21 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteNot Specified
P21 ARNKO P21 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Not Specified Conditional mutant.
P35 WT P35 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteNot Specified
P35 ARNKO P35 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Not Specified Conditional mutant.
10 weeks WT P w 10 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteNot Specified
10 weeks ARNKO P w 10 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Not Specified Conditional mutant.
15 weeks WT P w 15 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1ArteNot Specified
15 weeks ARNKO P w 15 TS28: hypothalamus Present 1 µg; total RNA involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6JBomTac Nampttm1.1Arte/Nampttm1.1Arte, Agrptm1(cre)Lowl/Agrp+Not Specified Conditional mutant.
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) No statistically significant differences between the two genotypes are observed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory