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Gene Expression Data
Assay Details
Assay
Reference: J:43443 Taniguchi M, et al., Disruption of semaphorin III/D gene causes severe abnormality in peripheral nerve projection. Neuron. 1997 Sep;19(3):519-30
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6761850
Gene symbol: Sema3a
Gene name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Reporter: lacZ
Direct Detection
Assay notes: Fixative was 2.5% paraformaldehyde/0.2% glutaraldehyde.
Results
Specimen 2A: embryonic day 10.5; Sema3atm1.3Tyag/Sema3a+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud Strong 2A
TS17: hindlimb bud Moderate 2A
TS17: somite Moderate Regionally restricted 2A Expression was predominantly in the caudal part of each somite.
TS17: 1st branchial arch Weak 2A
TS17: 2nd branchial arch Weak 2A
TS17: heart Weak 2A
TS17: head mesenchyme Weak Regionally restricted 2A Expression is in tissues surrounding the eye.
Specimen 2B: embryonic day 12.0; Sema3atm1.3Tyag/Sema3a+ (more )
Structure Level Pattern Image Note
TS20: forelimb Present 2B
TS20: heart Present 2B
TS20: hindlimb Present 2B
TS20: mandibular process Present 2B
TS20: pinna Present 2B
TS20: sclerotome Present 2B
TS20: tongue Present 2B
TS20: head Present Regionally restricted 2B Expression is in the first branchial arch.
TS20: nose Present Regionally restricted 2B Expression is in the nasal foramen.
TS20: head mesenchyme Weak Regionally restricted 2B Expression is in tissues surrounding the eye.
TS20: brain Weak 2B
TS20: eye Weak 2B
TS20: spinal cord Weak 2B
Specimen P: postnatal; Sema3atm1.3Tyag/Sema3a+ (more )
Structure Level Pattern Image Note
TS28: mouse Present Regionally restricted The authors state that expression was found in many of the same tissues as seen during embryogenesis.
Specimen P2: postnatal day 2; Sema3atm1.3Tyag/Sema3a+ (more )
Structure Level Pattern Image Note
TS27: cerebellum Purkinje cell layer Strong
Specimen 4C: embryonic day 13.0; Sema3atm1.3Tyag/Sema3a+ (more )
Note: Double labeled: blue - Sema3a reporter; brown - Nefm (assay MGI:6761855).
Structure Level Pattern Image Note
TS21: face Present 4C
Specimen 4D: embryonic day 13.0; Sema3atm1.3Tyag/Sema3atm1.3Tyag (more )
Note: Double labeled: blue - Sema3a reporter; brown - Nefm (assay MGI:6761855).
Structure Level Pattern Image Note
TS21: face Present 4D
Specimen 4I: embryonic day 13.0; Sema3atm1.3Tyag/Sema3a+ (more )
Structure Level Pattern Image Note
TS21: forelimb pre-cartilage condensation Present 4I
TS21: hand pre-cartilage condensation Present 4I
TS21: humerus cartilage condensation Present 4I
TS21: radius cartilage condensation Present 4I
TS21: radius-ulna cartilage condensation Present 4I
Specimen 4J: embryonic day 13.0; Sema3atm1.3Tyag/Sema3atm1.3Tyag (more )
Note: Double labeled: blue - Sema3a reporter; brown - Nefm (assay MGI:6761855).
Structure Level Pattern Image Note
TS21: forelimb pre-cartilage condensation Present 4J
TS21: hand pre-cartilage condensation Present 4J
TS21: humerus cartilage condensation Present 4J
TS21: radius cartilage condensation Present 4J
TS21: radius-ulna cartilage condensation Present 4J
Specimen 4M: embryonic day 13.0; Sema3atm1.3Tyag/Sema3a+ (more )
Note: Double labeled: blue - Sema3a reporter; brown - Nefm (assay MGI:6761855).
Structure Level Pattern Image Note
TS21: sclerotome Present 4M
Specimen not shown: embryonic day 9.5; Sema3atm1.3Tyag/Sema3a+ (more )
Structure Level Pattern Image Note
TS15: forelimb bud Present
TS15: somite Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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