Probe:	Dkk3 probe3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: cortical hem	Present		1D	Expression restricted to caudomedial margin of cortical neuroepithelium known as the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: cortical hem	Present		1E
TS20: thalamus	Present		1E	Expression in eminentia thalami.

Structure	Level	Pattern	Image	Note
TS20: cortical hem	Present		1E inset	Expression in hem broadens toward posterior end of hippocampal anlage.
TS20: thalamus	Present		1E inset	Expression in eminentia thalami.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	1F	Circumscribed expression in posterior strionuclear neuroepithelium and anterior thalamic neuroepithelium.
TS20: hypothalamus ventricular layer	Present	Regionally restricted	1F	Expression in ventricular zone of hypothalamus.

Structure	Level	Pattern	Image	Note
TS23: telencephalon subventricular zone	Present	Regionally restricted	2A	Expression in ventricular and subventricular zone of lateral ventricle.
TS23: telencephalon ventricular layer	Present	Regionally restricted	2A	Expression in ventricular and subventricular zone of lateral ventricle.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Strong	Regionally restricted	2B	Strong expression in neurons throughout cortex, predominantly in outer cortical layers.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Strong	Regionally restricted	2C	Strong expression in neurons throughout cortex, predominantly in outer cortical layers.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	2D	Strong expression in neurons throughout cortex, predominantly in outer cortical layers.
TS28: lateral ventricle ependyma	Present		2D	Expression in row of cells in wall of lateral ventricles.

Structure	Level	Pattern	Image	Note
TS23: telencephalon subventricular zone	Present	Regionally restricted	2E	Expression in ventricular and subventricular zone of lateral ventricle.
TS23: telencephalon ventricular layer	Present	Regionally restricted	2E	Expression in ventricular and subventricular zone of lateral ventricle.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		2F
TS25: hippocampus	Present		2F

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Present		2G
TS27: hippocampus	Present		2G

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2H
TS28: hippocampus CA1	Present	Regionally restricted	2H	Expression in pyramidal cells in CA1-3.
TS28: hippocampus CA2	Present	Regionally restricted	2H	Expression in pyramidal cells in CA1-3.
TS28: hippocampus CA3	Present	Regionally restricted	2H	Expression in pyramidal cells in CA1-3.
TS28: dentate gyrus granule cell layer	Absent		2H

Structure	Level	Pattern	Image	Note
TS23: lateral ventricle ependyma	Present		2I	Expression in ventricular and subventricular zone of lateral.
TS23: telencephalon subventricular zone	Present		2I	Expression in ventricular and subventricular zone of lateral.

Structure	Level	Pattern	Image	Note
TS23: 3rd ventricle ependyma	Present		2K	Expression in ventricular and subventricular zone of 3rd ventricle.
TS23: diencephalon subventricular zone	Present		2K	Expression in ventricular and subventricular zone of 3rd ventricle.
TS23: epithalamus	Present		2K
TS23: thalamus	Present	Regionally restricted	2K	Expression in proliferative neuroepithelium of thalamus.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Present		2L
TS25: cerebral cortex ventricular zone	Present		2L
TS25: ganglionic eminence	Present		2L

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular zone	Present		2M	Expression in cortical ventricular zone of lateral ventricle.
TS25: hippocampus ventricular layer	Present		2M
TS25: hippocampal formation	Present	Regionally restricted	2M	Expression in a row of migratory cells that delaminated from primary dentate neuroepithelium (dentate notch).

Structure	Level	Pattern	Image	Note
TS25: hippocampal formation	Present	Regionally restricted	2N	Expression in cells delaminating from dentate notch. Expression in first dentate migration.
TS25: dentate gyrus	Present		2N

Structure	Level	Pattern	Image	Note
TS25: thalamus ventricular layer	Present	Regionally restricted	2O	Expression in ventricular neuroepitheium of anterior thalamus. Expression in proliferative neuroepithelium of thalamus.
TS25: thalamic nucleus	Present	Regionally restricted	2O	Expression in cells that delaminate from neuroepithelium into anterior medial thalamic nucleus.

Structure	Level	Pattern	Image	Note
TS25: 3rd ventricle ependyma	Present		2P
TS25: thalamus	Present	Regionally restricted	2P	Expression in proliferative neuroepithelium of thalamus.

Structure	Level	Pattern	Image	Note
TS25: hippocampal formation	Present	Regionally restricted	2Q	Expression in cells delaminating from dentate notch. Expression in first dentate migration.

Structure	Level	Pattern	Image	Note
TS25: thalamus	Present	Regionally restricted	2R	Expression in proliferative neuroepithelium of thalamus.
TS25: diencephalon ventricular layer	Present	Regionally restricted	2R	Expression in ventricular zone of 3rd ventricle.
TS25: thalamic nucleus	Present	Regionally restricted	2R	Expression in cells that delaminate into anterior medial thalamic nucleus.

Structure	Level	Pattern	Image	Note
TS25: 3rd ventricle ependyma	Strong		2S
TS25: diencephalon subventricular zone	Strong		2S

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Present	Regionally restricted	2T	Expression in cells delaminating from dentate notch. Expression in second dentate migration.
TS27: dentate gyrus	Present	Regionally restricted	2T	Expression in secondary germinal matrix of dentate gyrus. Expression in outer layer of external and internal limbs of dentate gyrus.

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present	Regionally restricted	2U	Expression in densely packed neurons in cortical layers II-VI.
TS27: cortical layer III	Present	Regionally restricted	2U	Expression in densely packed neurons in cortical layers II-VI.
TS27: cortical layer IV	Present	Regionally restricted	2U	Expression in densely packed neurons in cortical layers II-VI.
TS27: cortical layer V	Present	Regionally restricted	2U	Expression in densely packed neurons in cortical layers II-VI.
TS27: cortical layer VI	Present	Regionally restricted	2U	Expression in densely packed neurons in cortical layers II-VI.

Structure	Level	Pattern	Image	Note
TS27: 3rd ventricle ependyma	Present		2V
TS27: diencephalon subventricular zone	Present		2V

Structure	Level	Pattern	Image	Note
TS28: hippocampus region	Present	Regionally restricted	2W	Expression in pyramidal neurons of CA fields.
TS28: dentate gyrus	Absent		2W

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	2X	Expression in row of cells in ventricular zone of lateral ventricle lining the putamen.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	2Y	Expression throughout cortex.

Structure	Level	Pattern	Image	Note
TS20: retina epithelium	Strong	Regionally restricted	4C	Strong expression in neuroepithelium of optic bulb. Strong expression in outer neuroblastic layer.
TS20: lens vesicle	Present		4C

Structure	Level	Pattern	Image	Note
TS23: retina epithelium	Strong	Regionally restricted	4F	Strong expression in outer neuroblastic layer. No detectable expression in inner neuroblastic layer.
TS23: lens	Present		4F

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		4G

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		6E W: dkk3

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		6E M: dkk3	No significant difference in expression between wild-type and mutant in hippocampal and thalamic structures.