About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:278916 Maruyama K, et al., Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development. Dev Biol. 2019 Aug 15;452(2):134-143
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6759001
Gene symbol: Prox1
Gene name: prospero homeobox 1
Reporter: GFP
Direct Detection
Assay notes: Expression of EGFP in Prox1<tm1.1Fuma> allele was activated by Cre.
Results
Specimen 1A: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1A merge , 1A eGFP Expressed extensively in cardiac lymphatic vessels (Tek-cre activated).
TS26: lymphatic vessel endothelium Present Regionally restricted 1A merge , 1A eGFP Expressed extensively in cardiac lymphatic vessels (Tek-cre activated).
Specimen 1B: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1B eGFP , 1B merge Expressed in hypoplastic cardiac lymphatic vessels (Tek-cre activated). Relatively preserved.
TS26: lymphatic vessel endothelium Present Regionally restricted 1B eGFP , 1B merge Expressed in hypoplastic cardiac lymphatic vessels (Tek-cre activated). Relatively preserved.
Specimen 1C: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1C merge , 1C eGFP Expressed extensively in cardiac lymphatic vessels (Tek-cre activated).
TS26: lymphatic vessel endothelium Present Regionally restricted 1C merge , 1C eGFP Expressed extensively in cardiac lymphatic vessels (Tek-cre activated).
Specimen 1D: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1D eGFP , 1D merge Expressed in hypoplastic cardiac lymphatic vessels (Tek-cre activated). Relatively preserved.
TS26: lymphatic vessel endothelium Present Regionally restricted 1D eGFP , 1D merge Expressed in hypoplastic cardiac lymphatic vessels (Tek-cre activated). Relatively preserved.
Specimen 1E: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1E eGFP , 1E merge Expressed extensively in cardiac lymphatic vessels (Tek-cre activated).
TS26: lymphatic vessel endothelium Present Regionally restricted 1E eGFP , 1E merge Expressed extensively in cardiac lymphatic vessels (Tek-cre activated).
Specimen 1F: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1F merge , 1F eGFP Expressed in hypoplastic cardiac lymphatic vessels (Tek-cre activated). Scarce lymphatic vessels on the dorsal ventricular wall.
TS26: lymphatic vessel endothelium Present Regionally restricted 1F merge , 1F eGFP Expressed in hypoplastic cardiac lymphatic vessels (Tek-cre activated). Scarce lymphatic vessels on the dorsal ventricular wall.
Specimen 3A/B: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3A , 3B Expressed in lymphatic vessels around the aorta and on the ventral side of the heart, but did not contribute to those on the dorsal side (Isl1-cre activated).
TS25: right ventricle cardiac muscle Present 3A , 3B Isl1-cre activated.
Specimen 3C/D: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Dorsal side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3C , 3D Expressed in lymphatic vessels around the aorta and on the ventral side of the heart, but did not contribute to those on the dorsal side (Isl1-cre activated).
TS25: right ventricle cardiac muscle Present 3C , 3D Isl1-cre activated.
Specimen 3E/F: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: right ventricle cardiac muscle Present 3E , 3F Isl1-cre activated. Lymphatic vessel formation was impaired particularly on the ventral side.
Specimen 3G/H: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Dorsal side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: right ventricle cardiac muscle Present 3H , 3G Isl1-cre activated.
Specimen 3I/J: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3I , 3J Merge , 3J eGFP Expressed in lymphatic vessels around the aorta on the ventral side of the heart (Isl1-cre activated).
Specimen 3K/L: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3L Merge , 3K , 3L eGFP Decreased expression in impaired lymphatic vessels particularly on the ventral side (Isl1-cre activated).
Specimen S1D fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1D left Expressed in cardiac lymphatic vessels (Tek-cre activated).
TS25: lymphatic vessel endothelium Present Regionally restricted S1D left Expressed in cardiac lymphatic vessels (Tek-cre activated).
Specimen S1D fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1D right Expressed in hypoplastic cardiac lymphatic vessels (Tek-cre activated). Relatively preserved.
TS25: lymphatic vessel endothelium Present Regionally restricted S1D right Expressed in hypoplastic cardiac lymphatic vessels (Tek-cre activated). Relatively preserved.
Specimen S1E/F fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1E left Expressed in endothelial cells (Tek-cre activated).
TS25: heart blood vessel Present Regionally restricted S1E left Expressed in endothelial cells (Tek-cre activated).
TS25: pulmonary artery Present Regionally restricted S1E left Expressed in endothelial cells (Tek-cre activated).
TS25: lymphatic vessel endothelium Present Regionally restricted S1F merge left , S1E left , S1F eGFP left Expressed in cardiac lymphatic vessels (Tek-cre activated).
Specimen S1E/F fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: green - Prox1; blue - Pecam1 (assay MGI:6758967); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1E right Expressed in endothelial cells (Tek-cre activated).
TS25: heart blood vessel Present Regionally restricted S1E right Expressed in endothelial cells (Tek-cre activated).
TS25: pulmonary artery Present Regionally restricted S1E right Expressed in endothelial cells (Tek-cre activated).
TS25: lymphatic vessel endothelium Present Regionally restricted S1F merge right , S1E right , S1F eGFP right Aberrant Lyve1-negative microvasculature was formed by Foxa1+/Pecam1+ cells around the aortic stem sites (Tek-cre activated).
Specimen S4A/B: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: face skin Present Regionally restricted S4A , S4B Expressed in the second heart field-derived facial tissues (Isl1-cre activated).
Specimen S4C: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. ventral side
Structure Level Pattern Image Note
TS25: outflow tract Present S4C Isl1-cre activated.
Specimen S4D: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. dorsal side
Structure Level Pattern Image Note
TS25: heart right ventricle Present S4D Isl1-cre activated.
Specimen S4G/H: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Triple labeled: green - Prox1; red - Prox1 (assay MGI:6758976); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: face skin Present Regionally restricted S4G , S4H eGFP , S4H merge Colocalized with Pecam1 and Prox1 in facial skin lymphatic vessels (Isl1-cre activated).
TS25: lymphatic vessel endothelium Present Regionally restricted S4G , S4H eGFP , S4H merge Colocalized with Pecam1 and Prox1 in facial skin lymphatic vessels (Isl1-cre activated).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory