About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:278916 Maruyama K, et al., Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development. Dev Biol. 2019 Aug 15;452(2):134-143
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6758996
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: anti-Prox1 (#11-002)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4G: embryonic day 9.5 (more )
Note: Triple labeled: green - Prox1; red - Isl1 (assay MGI:6758993); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS15: 1st branchial arch mesenchyme Present Clusters 4G Merge , 4G Prox1 Expressed in the Isl1+ core mesoderm.
TS15: 2nd branchial arch mesenchyme Present Clusters 4G Merge , 4G Prox1 Expressed in the Isl1+ core mesoderm.
Specimen 5A/B: embryonic day 9.5 (more )
Note: Left lateral. Triple labeled: green - Prox1; red - Flt4 (assay MGI:6758981); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS15: 1st branchial arch Present 5A Partially merged with Pecam1 and Flt4.
TS15: 2nd branchial arch Present Clusters 5B Merge , 5A , 5B Prox1 , 5B Prox1-VEGFR3 Partially merged with Pecam1 and Flt4.
Specimen 5C/D: embryonic day 10.5 (more )
Note: Left lateral. Triple labeled: green - Prox1; red - Flt4 (assay MGI:6758981); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS17: 1st branchial arch Present Clusters 5D Prox1 , 5D Merge , 5C , 5D Prox1-VEGFR3 Partially coexpressed with Flt4. Flt4+/Pecam1+ cells with and without Prox1 expression appeared to form mesh-like network structures, which extended caudally.
TS17: 2nd branchial arch Present Clusters 5D Prox1 , 5D Merge , 5C , 5D Prox1-VEGFR3 Partially coexpressed with Flt4. Flt4+/Pecam1+ cells with and without Prox1 expression appeared to form mesh-like network structures, which extended caudally.
Specimen 5E/F: embryonic day 12.5 (more )
Note: Ventral side. Triple labeled: green - Prox1; red - Flt4 (assay MGI:6758981); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 5F Prox1-VEGFR3 , 5E , 5F Prox1 , 5F Merge Mesh-like networks composed of Prox1+ and/or Flt4+ cells appeared, surrounding the outflow tract.
Specimen 5G/H: embryonic day 15.0 (more )
Note: Ventral side. Triple labeled: green - Prox1; red - Flt4 (assay MGI:6758981); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS23: lymphatic vessel endothelium Present Regionally restricted 5H Prox1 , 5H Prox1-VEGFR3 , 5H Merge , 5G Pecam1+/Prox1+/Flt4+ thick cardiac lymphatics were recognized on the ventral side of the aorta.
Specimen 5I/J: embryonic day 16.5 (more )
Note: Ventral side. Triple labeled: green - Prox1; red - Flt4 (assay MGI:6758981); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS24: lymphatic vessel endothelium Present Regionally restricted 5I , 5J Prox1 , 5J Merge , 5J Prox1-VEGFR3 Prox1+ cells around the outflow tract were remodeled and formed mature cardiac lymphatics.
Specimen 6A: embryonic day 12.0 (more )
Note: Left lateral. Triple labeled: green - Prox1; red - Lyve1 (assay MGI:6758952); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS19: 2nd branchial arch Present 6A Prox1-LYVE1 , 6A Prox1 , 6A Merge Mostly negative for Lyve1.
Specimen 6I: embryonic day 15.0 (more )
Note: Dorsal side. Triple labeled: green - Prox1; red - Flt4 (assay MGI:6758981); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS23: lymphatic vessel endothelium Present Regionally restricted 6I right , 6I left On the dorsal side of the hearts, Pecam1+/Prox1+/Flt4+ mature cardiac lymphatic vessels extended over the ventricular wall.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory