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Gene Expression Data
Assay Details
Assay
Reference: J:278916 Maruyama K, et al., Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development. Dev Biol. 2019 Aug 15;452(2):134-143
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6758976
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: anti-Prox1 (AF2727)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 2A/B: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; blue - Pecam1 (assay MGI:6758967); green - Cre reporter.
Structure Level Pattern Image Note
TS19: 2nd branchial arch Present 2A , 2B Colocalized with Isl1+ lineage.
Specimen 2C: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; blue - Pecam1 (assay MGI:6758967); green - Cre reporter.
Structure Level Pattern Image Note
TS19: heart atrium Present 2C Prox1 , 2C Merge
TS19: common cardinal vein Present 2C Merge , 2C Prox1 Not colocalized with Isl1+ lineage.
Specimen 2D/E: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; blue - Pecam1 (assay MGI:6758967); green - Cre reporter.
Structure Level Pattern Image Note
TS21: lymphatic system Present Regionally restricted 2D , 2E Colocalized with Isl1+ lineage around the aorta.
Specimen 2F/G: embryonic day 17.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; blue - Pecam1 (assay MGI:6758967); green - Cre reporter.
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 2F , 2G Colocalized with Isl1+ lineage around the outflow tract.
Specimen S2B/C: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; blue - Pecam1 (assay MGI:6758967); green - Cre reporter.
Structure Level Pattern Image Note
TS21: lymph sac Present Regionally restricted S2B , S2C merge , S2C Prox1 Many cardiac lymphatic endothelial cells lining the jugular lymph sacs were Isl1+ lineage.
Specimen S4G/H: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: face skin Present Regionally restricted S4G , S4H Prox1 , S4H merge Expressed in facial skin lymphatic vessels.
TS25: lymphatic vessel endothelium Present Regionally restricted S4G , S4H Prox1 , S4H merge Expressed in facial skin lymphatic vessels.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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