Nanog Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:191065 Kang M, et al., FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse. Development. 2013 Jan;140(2):267-79
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6756421
Gene symbol:	Nanog
Gene name:	Nanog homeobox

Antibody:	anti-NANOG
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

1A ©

1A'' ©

Specimen 1A': embryonic day 2.5 (more

)
Note: 21 cells, late morula embryo. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1A , 1A''	Expression is in the nuclei of the majority of inner cell mass cells.

1B ©

1B'' ©

Specimen 1B': embryonic day 2.5; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

)
Note: 21 cells, late morula embryo. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4^tm1Gol instead of Fgf4^tm1.3Mrt. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1B , 1B''	Expression in this mutant is the same as in wild-type.

1C ©

1C'' ©

Specimen 1C': embryonic day 3.0 (more

)
Note: 35 cells, early blastocyst. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1C'' , 1C	Expression is in the nuclei of the majority of inner cell mass cells.

1D ©

1D'' ©

Specimen 1D': embryonic day 3.0; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

)
Note: 35 cells, early blastocyst. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4^tm1Gol instead of Fgf4^tm1.3Mrt. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1D'' , 1D	Expression in this mutant is the same as in wild-type.

1E ©

1E'' ©

Specimen 1E': embryonic day 3.5 (more

)
Note: 51 cells, early blastocyst. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	1E'' , 1E	Expression in the epiblast.

1F ©

1F'' ©

Specimen 1F': embryonic day 3.5; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

)
Note: 56 cells, early blastocyst. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4^tm1Gol instead of Fgf4^tm1.3Mrt. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		1F , 1F''	Expression is in the entire inner cell mass. The primitive endoderm layer is missing in this mutant.

1G ©

1G'' ©

Specimen 1G': embryonic day 4.0 (more

)
Note: 80 cells, mid blastocyst. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	1G , 1G''	Expression in the epiblast.

1H ©

1H'' ©

Specimen 1H': embryonic day 4.0; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

)
Note: 84 cells, mid blastocyst.The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4^tm1Gol instead of Fgf4^tm1.3Mrt. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		1H , 1H''	Expression is in the entire inner cell mass. The primitive endoderm layer is missing in this mutant.

1I ©

1I'' ©

Specimen 1I': embryonic day 4.5 (more

)
Note: >100cells, late blastocyst. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	1I'' , 1I	Expression in the epiblast.

1J ©

1J'' ©

Specimen 1J': embryonic day 4.5; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

)
Note: The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4^tm1Gol instead of Fgf4^tm1.3Mrt. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		1J , 1J''	Expression is in the entire inner cell mass. The primitive endoderm layer is missing in this mutant.

2A zFgf4+/+ Merge ©

2A zFgf4+/+ NANOG ©

Specimen 2A zFgf4+/+: embryonic day 2.0 (more

)
Note: 15 cell, morula stage embryo. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		2A zFgf4+/+ NANOG , 2A zFgf4+/+ Merge

2A mFgf4-/-; zFgf4+/- Merge ©

2A mFgf4-/-; zFgf4+/- NANOG ©

Specimen 2A mFgf4-/-; zFgf4+/- E2: embryonic day 2.0; Fgf4^tm1.2Mrt/Fgf4⁺, Tg(Msx2-cre)5Rem/0 (more

)
Note: Conditional mutant. 16 cells, morula stage. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		2A mFgf4-/-; zFgf4+/- Merge , 2A mFgf4-/-; zFgf4+/- NANOG

2A mFgf4-/-; zFgf4-/- Merge ©

2A mFgf4-/-; zFgf4-/- NANOG ©

Specimen 2A mFgf4-/-; zFgf4-/- E2: embryonic day 2.0; Fgf4^tm1.2Mrt/Fgf4^tm1.3Mrt, Tg(Zp3-cre)3Mrt/0 (more

)
Note: Conditional mutant. 16 cells, morula stage. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		2A mFgf4-/-; zFgf4-/- NANOG , 2A mFgf4-/-; zFgf4-/- Merge

2A zFgf4-/- Merge ©

2A zFgf4-/- NANOG ©

Specimen 2A zFGF4-/-: embryonic day 2.0; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

)
Note: 14 cells, morula stage. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4^tm1Gol instead of Fgf4^tm1.3Mrt. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		2A zFgf4-/- Merge , 2A zFgf4-/- NANOG

2B mFgf4-/-; zFgf4+/- E3.0 Merge 2D ©

2B mFgf4-/-; zFgf4+/- E3.0 Merge 3D ©

2B mFgf4-/-; zFgf4+/- E3.0 NANOG ©

Specimen 2B mFgf4-/-; zFgf4+/- E3: embryonic day 3.0; Fgf4^tm1.2Mrt/Fgf4⁺, Tg(Msx2-cre)5Rem/0 (more

)
Note: Conditional mutant. 32 cells. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		2B mFgf4-/-; zFgf4+/- E3.0 Merge 2D , 2B mFgf4-/-; zFgf4+/- E3.0 Merge 3D , 2B mFgf4-/-; zFgf4+/- E3.0 NANOG

2B mFgf4-/-; zFgf4+/- E3.5 Merge 2D ©

2B mFgf4-/-; zFgf4+/- E3.5 Merge 3D ©

2B mFgf4-/-; zFgf4+/- E3.5 NANOG ©

Specimen 2B mFgf4-/-; zFgf4+/- E3.5: embryonic day 3.5; Fgf4^tm1.2Mrt/Fgf4⁺, Tg(Msx2-cre)5Rem/0 (more

)
Note: Conditional mutant. 52 cells. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		2B mFgf4-/-; zFgf4+/- E3.5 Merge 2D , 2B mFgf4-/-; zFgf4+/- E3.5 Merge 3D , 2B mFgf4-/-; zFgf4+/- E3.5 NANOG

2B mFgf4-/-; zFgf4-/- E3.0 Merge 2D ©

2B mFgf4-/-; zFgf4-/- E3.0 Merge 3D ©

2B mFgf4-/-; zFgf4-/- E3.0 NANOG ©

Specimen 2B mFgf4-/-; zFgf4-/- E3: embryonic day 3.0; Fgf4^tm1.2Mrt/Fgf4^tm1.3Mrt, Tg(Zp3-cre)3Mrt/0 (more

)
Note: Conditional mutant. 32 cells. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		2B mFgf4-/-; zFgf4-/- E3.0 Merge 3D , 2B mFgf4-/-; zFgf4-/- E3.0 NANOG , 2B mFgf4-/-; zFgf4-/- E3.0 Merge 2D

2B mFgf4-/-; zFgf4-/- E3.5 Merge 2D ©

2B mFgf4-/-; zFgf4-/- E3.5 Merge 3D ©

2B mFgf4-/-; zFgf4-/- E3.5 NANOG ©

Specimen 2B mFgf4-/-; zFgf4-/- E3.5: embryonic day 3.5; Fgf4^tm1.2Mrt/Fgf4^tm1.3Mrt, Tg(Zp3-cre)3Mrt/0 (more

)
Note: Conditional mutant. 59 cells. Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		2B mFgf4-/-; zFgf4-/- E3.5 Merge 3D , 2B mFgf4-/-; zFgf4-/- E3.5 NANOG , 2B mFgf4-/-; zFgf4-/- E3.5 Merge 2D

3A Fgf4+/+ E3.5 Merge ©

3A Fgf4+/+ E3.5 NANOG ©

Specimen 3A Fgf4+/+ E3.5: embryonic day 3.5 (more

)
Note: 60 cells. Double labeled: white - Nanog; red - Sox17 (assay MGI:6756434).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		3A Fgf4+/+ E3.5 Merge , 3A Fgf4+/+ E3.5 NANOG

3A Fgf4-/- E3.5 Merge ©

3A Fgf4-/- E3.5 NANOG ©

Specimen 3A Fgf4-/- E3.5: embryonic day 3.5; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

)
Note: 58 cells. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4^tm1Gol instead of Fgf4^tm1.3Mrt. Double labeled: white - Nanog; red - Sox17 (assay MGI:6756434).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		3A Fgf4-/- E3.5 NANOG , 3A Fgf4-/- E3.5 Merge

Specimen 3A Fgf4+/+ E4.5: embryonic day 4.5 (more

)
Note: 92 cells. Double labeled: white - Nanog; red - Sox17 (assay MGI:6756434).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		3A Fgf4+/+ E4.5 Merge , 3A Fgf4+/+ E4.5 NANOG

Specimen 3A Fgf4-/- E4.5: embryonic day 4.5; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

)
Note: 72 cells. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4^tm1Gol instead of Fgf4^tm1.3Mrt.Double labeled: white - Nanog; red - Sox17 (assay MGI:6756434).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		3A Fgf4-/- E4.5 Merge , 3A Fgf4-/- E4.5 NANOG

Specimen 3B Fgf4+/+ E3.5: embryonic day 3.5 (more

)
Note: 67 cells. Double labeled: white - Nanog; red - Gata4 (assay MGI:6756436).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		3B Fgf4+/+ E3.5 Merge

Specimen 3B Fgf4-/- E3.5: embryonic day 3.5; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

)
Note: 65 cells. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4^tm1Gol instead of Fgf4^tm1.3Mrt. Double labeled: white - Nanog; red - Gata4 (assay MGI:6756436).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		3B Fgf4-/- E3.5 Merge , 3B Fgf4-/- E3.5 NANOG

Specimen 3B Fgf4+/+ E4.5: embryonic day 4.5 (more

)
Note: 80 cells. Double labeled: white - Nanog; red - Gata4 (assay MGI:6756436).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		3B Fgf4+/+ E4.5 Merge

Specimen 3B Fgf4-/- E4.5: embryonic day 4.5; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		3B Fgf4-/- E4.5 NANOG , 3B Fgf4-/- E4.5 Merge

Specimen S4A Fgf4+/+: embryonic day 3.5 (more

)
Note: Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		S4A Fgf4+/+ Merge , S4A Fgf4+/+ NANOG

Specimen S4A Fgf4-/-: embryonic day 3.5; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Strong		S4A Fgf4-/- NANOG , S4A Fgf4-/- Merge

Specimen S4C Fgf4+/+: embryonic day 4.5 (more

)
Note: Double labeled: white - Nanog; red - Gata6 (assay MGI:6756341).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		S4C Fgf4+/+ Merge , S4C Fgf4+/+ NANOG

Specimen S4C Fgf4-/-: embryonic day 4.5; Fgf4^tm1.3Mrt/Fgf4^tm1.3Mrt (more

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Strong		S4C Fgf4-/- NANOG , S4C Fgf4-/- Merge

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24