About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:191065 Kang M, et al., FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse. Development. 2013 Jan;140(2):267-79
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6756436
Gene symbol: Gata4
Gene name: GATA binding protein 4
Antibody: anti-GATA4
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3B Fgf4+/+ E3.5: embryonic day 3.5 (more )
Note: 67 cells.
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B Fgf4+/+ E3.5 Merge

Specimen 3B Fgf4-/- E3.5: embryonic day 3.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: 65 cells. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Gata4; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Absent 3B Fgf4-/- E3.5 GATA4 , 3B Fgf4-/- E3.5 Merge

Specimen 3B Fgf4+/+ E4.5: embryonic day 4.5 (more )
Note: 80 cells.
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B Fgf4+/+ E4.5 Merge

Specimen 3B Fgf4-/- E4.5: embryonic day 4.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: 91 cells. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Gata4; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Absent 3B Fgf4-/- E4.5 Merge , 3B Fgf4-/- E4.5 GATA4


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/25/2025
MGI 6.24
The Jackson Laboratory