Gene Expression Data
Assay Details
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Assay
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Reference:
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J:297659
Ge Y, et al., Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. Dev Dyn. 2020 Aug 2;
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6750868
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Gene symbol:
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Atoh7
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Gene name:
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atonal bHLH transcription factor 7
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Reporter:
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ZsGreen
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Direct Detection
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Results
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Specimen
1C:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm3(cre/ERT2)Xmu/Atoh7+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2A:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm3(cre/ERT2)Xmu/Atoh7+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neural retina |
Present |
Regionally restricted |
2A
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Expression is seen in a subset of retinal progenitor cells in the central retina region.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm3(cre/ERT2)Xmu/Atoh7+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Widespread |
2B
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Expression is seen in a subset of retinal progenitor cells; 63% of expressing cells are found in the neuroblast layer, 37% are found in the inner ganglion cell layer. Expression in the GCL is believed to be due to the stability of the zsGreen protein.
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm3(cre/ERT2)Xmu/Atoh7+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: neural retina |
Present |
Regionally restricted |
2C
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Expression is primarily seen in differentiated cells in the ganglion cell layer and photoreceptors of the future outer nuclear layer. Expression is markedly reduced in the neuroblast layer.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm3(cre/ERT2)Xmu/Atoh7+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: photoreceptor layer |
Present |
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2D
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Expression is primarily seen in differentiated cells in the ganglion cell layer and photoreceptors of the future outer nuclear layer. Expression is markedly reduced in the neuroblast layer.
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| TS27: retina ganglion cell layer |
Present |
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2D
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Expression is primarily seen in differentiated cells in the ganglion cell layer and photoreceptors of the future outer nuclear layer. Expression is markedly reduced in the neuroblast layer.
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| TS27: retina outer nuclear layer |
Present |
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2D
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Expression is primarily seen in differentiated cells in the ganglion cell layer and photoreceptors of the future outer nuclear layer. Expression is markedly reduced in the neuroblast layer.
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| TS27: neural retina |
Present |
Single cells |
2D
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Expression is primarily seen in differentiated cells in the ganglion cell layer and photoreceptors of the future outer nuclear layer. Expression is markedly reduced in the neuroblast layer.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm3(cre/ERT2)Xmu/Atoh7+
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2E/F/G:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm1.1Xmu/Atoh7tm3(cre/ERT2)Xmu
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Atoh7 ZsGreen reporter; red - Atoh7 HA tag (assay MGI:6750898).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Widespread |
2E'
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2E
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2G'
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2G
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Expression is seen in a subset of retinal progenitor cells; 63% of expressing cells are found in the neuroblast layer, 37% are found in the inner ganglion cell layer. Very few cells are found expressing the native protein in the inner ganglion cell layer, suggesting that the reporter expression seen there is due to the longenvity of the ZsGreen protein.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm1Gan/Atoh7tm3(cre/ERT2)Xmu
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Strong |
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2H
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The ganglion cell layer does not form in these mutants.
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Specimen
4A/B/C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm3(cre/ERT2)Xmu/Atoh7+
Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Atoh7; red - Pou4f2 (assay MGI:6750871).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Regionally restricted |
4C'
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4A'
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4C
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4A
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Expression is primarily seen in the neuroblast layer.
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Specimen
4D/E/F:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm3(cre/ERT2)Xmu/Atoh7+
Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Dividing cells also labeled for EdU (red).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Regionally restricted |
4D
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4F
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Expression is seen in cells in the neuroblast layer; EdU labeling shows that 28% of those cells are still in S phase.
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