Pou4f2^tm2Xmu
Targeted Allele Detail

Summary

• Symbol: Pou4f2^tm2Xmu
• Name: POU domain, class 4, transcription factor 2; targeted mutation 2, Xiuqian Mu
• MGI ID: MGI:6727036
• Synonyms: Pou4f2^FlagtdTomato
• Gene: Pou4f2 Location: Chr8:79159639-79163274 bp, - strand Genetic Position: Chr8, 36.99 cM, cytoband F1-F5
• Alliance: Pou4f2^tm2Xmu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:297659
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The FLAG-T2A-tdTomato-SV40 poly(A)-LoxP-Neo-Lox cassette was inserted in frame after the last codon of Pou4f2 by homologous recombination in the ES cells. It is a null allele designed to express both zsGreen and CreERT2 from the Atoh7 promoter. It is designed as a functional wild-type allele expressing a FLAG-tagged version of Pou4f2 and tdTomato from the Pou4f2 promoter (J:297659)

Expression

In Mice Carrying this Mutation:

18 assay results 2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pou4f2 Mutation: 7 strains or lines available

References

• Original: J:297659 Ge Y, et al., Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. Dev Dyn. 2020 Aug 2
• All: 2 reference(s)