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Gene Expression Data
Assay Details
Assay
Reference: J:180770 Bjerknes M, et al., Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol. 2012 Feb 15;362(2):194-218
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6741111
Gene symbol: Mki67
Gene name: antigen identified by monoclonal antibody Ki 67
Antibody: anti-Ki67 (TEC-3; M7249)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 11A: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Mki67; orange - Dclk1 (assay MGI:6741049); green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 11A-3 , 11A-1 Mki67+ Gfi1b+ Dclk1- brush cells were shown.
TS28: intestine epithelium Present 11A-3 , 11A-1 Mki67+ Gfi1b+ Dclk1- brush cells were shown.
Specimen 11B: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Mki67; orange - Dclk1 (assay MGI:6741049); green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 11B-3 , 11B-1 Mki67- Gfi1b+ Dclk1+ brush cells were shown.
TS28: intestine epithelium Present 11B-3 , 11B-1 Mki67- Gfi1b+ Dclk1+ brush cells were shown.
Specimen 11C: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Mki67; orange - Dclk1 (assay MGI:6741049); green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Weak 11C-1 , 11C-3 Rare Mki67+ Gfi1b+ Dclk1+ brush cells were shown.
TS28: intestine epithelium Weak 11C-1 , 11C-3 Rare Mki67+ Gfi1b+ Dclk1+ brush cells were shown.
Specimen 13A: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Mki67; green - Gfi1b (assay MGI:6741116); orange - Neurog3 (assay MGI:6741112).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13A-1 , 13A-3 Mki67+ Gfi1b+ Neurog3- brush cells were shown.
TS28: intestine epithelium Present 13A-1 , 13A-3 Mki67+ Gfi1b+ Neurog3- brush cells were shown.
Specimen 13B: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Mki67; green - Gfi1b (assay MGI:6741116); orange - Neurog3 (assay MGI:6741112).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13B-3 , 13B-1 Mki67+ Gfi1b+ Neurog3- brush cells were shown.
TS28: intestine epithelium Present 13B-3 , 13B-1 Mki67+ Gfi1b+ Neurog3- brush cells were shown.
Specimen 13C: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Mki67; green - Gfi1b (assay MGI:6741116); orange - Atoh1 (assay MGI:6741099).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13C-1 , 13C-3 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
TS28: intestine epithelium Present 13C-1 , 13C-3 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
Specimen 13D: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Mki67; green - Gfi1b (assay MGI:6741116); orange - Atoh1 (assay MGI:6741099).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13D-1 , 13D-3 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
TS28: intestine epithelium Present 13D-1 , 13D-3 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
Specimen 13E: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Mki67; green - Gfi1b (assay MGI:6741116); orange - Atoh1 (assay MGI:6741099).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13E-3 , 13E-1 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
TS28: intestine epithelium Present 13E-3 , 13E-1 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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