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Gene Expression Data
Assay Details
Assay
Reference: J:180770 Bjerknes M, et al., Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol. 2012 Feb 15;362(2):194-218
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6741049
Gene symbol: Dclk1
Gene name: doublecortin-like kinase 1
Antibody: Anti-DCAMKL1
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 2: postnatal (more )
Structure Level Pattern Image Note
TS28: intestine epithelium Present 2_Dclk1 Expressed in brush cells, provided the best signal to noise ratio.
Specimen 5: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: orange - Dclk1; green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 5_5-1 , 5_5-3 Co-expressed with Gfi1b in brush cells.
TS28: intestine epithelium Present 5_5-1 , 5_5-3 Co-expressed with Gfi1b in brush cells.
Specimen 7C: postnatal (more )
Structure Level Pattern Image Note
TS28: intestinal villus Present 7C Expressed in the brush cells, exhibiting an increasing differentiation gradient from the crypt base to the crypt top as demonstrated by increasing staining intensity and cell size.
TS28: intestine epithelium Present 7C Expressed in the brush cells, exhibiting an increasing differentiation gradient from the crypt base to the crypt top as demonstrated by increasing staining intensity and cell size.
Specimen 7D: postnatal (more )
Note: Triple labeled: orange - Dclk1; red - lectin UEA-I; purple - Insm1 (assay MGI:6741068).
Structure Level Pattern Image Note
TS28: intestinal villus Present 7D Expressed in the brush cells, exhibiting an increasing differentiation gradient from the crypt base to the crypt top as demonstrated by increasing staining intensity, accumulating UEA-I binding and cell size.
TS28: intestine epithelium Present 7D Expressed in the brush cells, exhibiting an increasing differentiation gradient from the crypt base to the crypt top as demonstrated by increasing staining intensity, accumulating UEA-I binding and cell size.
Specimen 9: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: orange - Dclk1; green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 9_5-1 , 9_5-3 Expressed in brush cells.
TS28: intestine epithelium Present 9_5-1 , 9_5-3 Expressed in brush cells.
Specimen 11A: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: orange - Dclk1; red - Mki67 (assay MGI:6741111); green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Present Regionally restricted 11A-4 , 11A-1 Not expressed in Gfi1b+ Mki67+ brush cells.
TS28: intestine epithelium Present Regionally restricted 11A-4 , 11A-1 Not expressed in Gfi1b+ Mki67+ brush cells.
Specimen 11B: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: orange - Dclk1; red - Mki67 (assay MGI:6741111); green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Present Regionally restricted 11B-4 , 11B-1 Co-expressed with Gfi1b in brush cells (Mki67-).
TS28: intestine epithelium Present Regionally restricted 11B-4 , 11B-1 Co-expressed with Gfi1b in brush cells (Mki67-).
Specimen 11C: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: orange - Dclk1; red - Mki67 (assay MGI:6741111); green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Weak Regionally restricted 11C-4 , 11C-1 Co-expressed with Gfi1b in brush cells (rare examples of Mki67+ Dclk1+).
TS28: intestine epithelium Weak Regionally restricted 11C-4 , 11C-1 Co-expressed with Gfi1b in brush cells (rare examples of Mki67+ Dclk1+).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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