About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:180770 Bjerknes M, et al., Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol. 2012 Feb 15;362(2):194-218
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6741099
Gene symbol: Atoh1
Gene name: atonal bHLH transcription factor 1
Antibody: anti-MATH1
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 4 left: postnatal (more )
Note: Triple labeled: orange - Atoh1; red - lectin UEA-I; purple - Insm1 (assay MGI:6741068).
Structure Level Pattern Image Note
TS28: intestinal villus Present 4_1 , 4_7 Expressed in enteroendocrine cells/granulocytes nuclei.
TS28: intestine epithelium Present 4_1 , 4_7 Expressed in enteroendocrine cells/granulocytes nuclei.
Specimen 4 right: postnatal (more )
Note: Triple labeled: orange - Atoh1; red - lectin UEA-I; purple - Insm1 (assay MGI:6741068).
Structure Level Pattern Image Note
TS28: intestinal villus Present 4_2 , 4_8 Expressed in mucous cells/granulocytes nuclei.
TS28: intestine epithelium Present 4_2 , 4_8 Expressed in mucous cells/granulocytes nuclei.
Specimen 6: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: orange - Atoh1; green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 6_6 , 6_2 Expressed in mucous cells (Gfi1b-).
TS28: intestine epithelium Present 6_6 , 6_2 Expressed in mucous cells (Gfi1b-).
Specimen 7B: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: orange - Atoh1; green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 7B Expressed in granulocytic lineage cells.
TS28: intestine epithelium Present 7B Expressed in granulocytic lineage cells.
Specimen 13C: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: orange - Atoh1; red - Mki67 (assay MGI:6741111); green - Gfi1b (assay MGI:67411116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13C-1 , 13C-4 Expressed in granulocytic lineage cells. Mki67+ Gfi1b+ Atoh1- brush cells were shown.
TS28: intestine epithelium Present 13C-1 , 13C-4 Expressed in granulocytic lineage cells. Mki67+ Gfi1b+ Atoh1- brush cells were shown.
Specimen 13D: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: orange - Atoh1; red - Mki67 (assay MGI:6741111); green - Gfi1b (assay MGI:67411116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13D-4 , 13D-1 Expressed in granulocytic lineage cells. Mki67+ Gfi1b+ Atoh1- brush cells were shown.
TS28: intestine epithelium Present 13D-4 , 13D-1 Expressed in granulocytic lineage cells. Mki67+ Gfi1b+ Atoh1- brush cells were shown.
Specimen 13E: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: orange - Atoh1; red - Mki67 (assay MGI:6741111); green - Gfi1b (assay MGI:67411116).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13E-4 , 13E-1 Expressed in granulocytic lineage cells. Mki67+ Gfi1b+ Atoh1- brush cells were shown.
TS28: intestine epithelium Present 13E-4 , 13E-1 Expressed in granulocytic lineage cells. Mki67+ Gfi1b+ Atoh1- brush cells were shown.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory