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Gene Expression Data
Assay Details
Assay
Reference: J:293793 Basta JM, et al., The core SWI/SNF catalytic subunit Brg1 regulates nephron progenitor cell proliferation and differentiation. Dev Biol. 2020 Aug 15;464(2):176-187
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6740175
Gene symbol: Smarca4
Gene name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Antibody: anti-Brg1 [EPNCIR11A] (ab110641)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: green - Brg1 (Smarca4); red - Ncam1 (assay MGI:6740176); blue - DAPI.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 3A Brg1flx/flx Expression in all compartments of developing kidney including cap mesenchyme, differentiating nephron structures, stroma and ureteric bud.
TS22: early nephron Present 3A Brg1flx/flx Expression in all compartments of developing kidney including cap mesenchyme, differentiating nephron structures, stroma and ureteric bud.
TS22: renal interstitium Present 3A Brg1flx/flx Expression in all compartments of developing kidney including cap mesenchyme, differentiating nephron structures, stroma and ureteric bud.
TS22: ureteric tip Present 3A Brg1flx/flx Expression in all compartments of developing kidney including cap mesenchyme, differentiating nephron structures, stroma and ureteric bud.
Specimen 3A Six2Cre Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: green - Brg1 (Smarca4); red - Ncam1 (assay MGI:6740176); blue - DAPI.
Structure Level Pattern Image Note
TS22: cap mesenchyme Absent 3A Six2Cre Brg1flx/flx Expression lost in cap mesenchyme surrounding ureteric bud tips, and its derivatives in developing nephrons.
TS22: early nephron Absent 3A Six2Cre Brg1flx/flx Expression lost in cap mesenchyme surrounding ureteric bud tips, and its derivatives in developing nephrons.
TS22: renal interstitium Present 3A Six2Cre Brg1flx/flx Expression retained in stroma and ureteric buds.
TS22: ureteric tip Present 3A Six2Cre Brg1flx/flx Expression retained in stroma and ureteric buds.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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