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Gene Expression Data
Assay Details
Assay
Reference: J:293793 Basta JM, et al., The core SWI/SNF catalytic subunit Brg1 regulates nephron progenitor cell proliferation and differentiation. Dev Biol. 2020 Aug 15;464(2):176-187
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6740176
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Antibody: clone NCAM-OB11 (C9672)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 3A Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: red - Ncam1; green - Brg1 (Smarca4 assay MGI:6740175); blue - DAPI.
Structure Level Pattern Image Note
TS22: renal vesicle Present 3A Brg1flx/flx
Specimen 3A Six2Cre Brg1flx/flx: embryonic day 14.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Ncam1; green - Brg1 (Smarca4 assay MGI:6740175); blue - DAPI.
Structure Level Pattern Image Note
TS22: renal vesicle Present 3A Six2Cre Brg1flx/flx
Specimen 5A Lef1/NCAM Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: red - Ncam1; green - Lef1 (assay MGI:6740179); blue - DAPI.
Structure Level Pattern Image Note
TS24: renal vesicle Present 5A Brg1flx/flx Lef1/NCAM
Specimen 5A Lef1/NCAM Six2Cre Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Ncam1; green - Lef1 (assay MGI:6740179); blue - DAPI.
Structure Level Pattern Image Note
TS24: renal vesicle Weak Regionally restricted 5A Six2Cre Brg1flx/flx Lef1/NCAM Authors report a 50% reduction in Lef1/NCAM+ differentiating structures.
Specimen 5A Wt1/NCAM Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn (more )
Note: Triple labeled: red - Ncam1; green - Wt1 (assay MGI:6740181); blue - DAPI.
Structure Level Pattern Image Note
TS24: renal vesicle Present 5A Brg1flx/flx Wt1/NCAM
Specimen 5A Wt1/NCAM Six2Cre Brg1flx/flx: embryonic day 16.5; Smarca4tm1.2Pcn/Smarca4tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: red - Ncam1; green - Wt1 (assay MGI:6740181); blue - DAPI.
Structure Level Pattern Image Note
TS24: renal vesicle Present 5A Six2Cre Brg1flx/flx Wt1/NCAM
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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