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Gene Expression Data
Assay Details
Assay
Reference: J:296652 Chan WH, et al., Differences in CART expression and cell cycle behavior discriminate sympathetic neuroblast from chromaffin cell lineages in mouse sympathoadrenal cells. Dev Neurobiol. 2016 Feb;76(2):137-49
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6726291
Gene symbol: Pnmt
Gene name: phenylethanolamine-N-methyltransferase
Antibody: anti-PNMT
Detection system: Secondary antibody coupled to Alexa Fluor 647
Assay notes: Fixative was Zamboni's fixative (2% formaldehyde and 15% saturated picric acid).
Results
Specimen 2H: embryonic day 12.5 (more )
Note: Triple labeled: magenta - Pnmt; green - Th (assay MGI:6726288); red - Cartpt; (assay MGI:6726289).
Structure Level Pattern Image Note
TS20: adrenal primordium Absent 2H , 2E
Specimen 2L: embryonic day 13.5 (more )
Note: Triple labeled: magenta - Pnmt; green - Th (assay MGI:6726288); red - Cartpt; (assay MGI:6726289).
Structure Level Pattern Image Note
TS21: adrenal gland Absent 2I , 2L
Specimen 2P: embryonic day 14.5 (more )
Note: Triple labeled: magenta - Pnmt; green - Th (assay MGI:6726288); red - Cartpt; (assay MGI:6726289).
Structure Level Pattern Image Note
TS22: adrenal medulla Present Regionally restricted 2M , 2P Expression is in chromaffin cells.
Specimen 2T: embryonic day 15.5 (more )
Note: Triple labeled: magenta - Pnmt; green - Th (assay MGI:6726288); red - Cartpt; (assay MGI:6726289).
Structure Level Pattern Image Note
TS23: adrenal medulla Present Regionally restricted 2Q , 2T Expression is in chromaffin cells.
Specimen 2X: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Pnmt; green - Th (assay MGI:6726288); red - Cartpt; (assay MGI:6726289).
Structure Level Pattern Image Note
TS24: adrenal medulla Present Regionally restricted 2X , 2U Expression is in chromaffin cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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