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Gene Expression Data
Assay Details
Assay
Reference: J:256775 Zhang H, et al., Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls. Circ Res. 2016 Jun 10;118(12):1880-93
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6723865
Gene symbol: Npr3
Gene name: natriuretic peptide receptor 3
Reporter: GFP
Antibody: anti-GFP (Nacalai)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3D: embryonic day 10.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Cdh5 (assay MGI:6723847).
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present 3D merge , 3D1 green , 3D green , 3D1 merge
TS17: atrium endocardial lining Present 3D2 green , 3D merge , 3D green , 3D2 merge
TS17: sinus venosus Absent 3D merge , 3D2 merge , 3D green , 3D2 green
Specimen 3E: embryonic day 12.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Pecam1 (assay MGI:6723863).
Structure Level Pattern Image Note
TS20: interventricular septum endocardial lining Present Regionally restricted 3E merge , 3E green Expression is seen in the endocardium but not in coronary vascular endothelial cells in the compact myocardium.
TS20: right ventricle endocardial lining Present Regionally restricted 3E merge , 3E green Expression is seen in the endocardium but not in coronary vascular endothelial cells in the compact myocardium.
TS20: left ventricle endocardial lining Present Regionally restricted 3E green inset , 3E merge inset , 3E merge , 3E green Expression is seen in the endocardium but not in coronary vascular endothelial cells in the compact myocardium.
Specimen 3F: embryonic day 14.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Pecam1 (assay MGI:6723863).
Structure Level Pattern Image Note
TS22: interventricular septum endocardial lining Present Regionally restricted 3F green , 3F merge Expression is seen in the endocardium but not in coronary vascular endothelial cells in the compact myocardium.
TS22: right ventricle endocardial lining Present Regionally restricted 3F green , 3F merge Expression is seen in the endocardium but not in coronary vascular endothelial cells in the compact myocardium.
TS22: left ventricle endocardial lining Present Regionally restricted 3F merge , 3F merge inset , 3F green inset , 3F green Expression is seen in the endocardium but not in coronary vascular endothelial cells in the compact myocardium.
Specimen Online 3B - upper: embryonic day 8.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Cdh5 (assay MGI:6723847).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3B upper green , Online 3B upper merge , Online 3B upper inset
Specimen Online 3B - center: embryonic day 8.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Cdh5 (assay MGI:6723847).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3B center merge , Online 3B center inset , Online 3B center green
TS13: common atrial chamber endocardial tube Present Online 3B center merge , Online 3B center green
Specimen Online 3B - lower: embryonic day 8.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Cdh5 (assay MGI:6723847).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3B lower green , Online 3B lower inset , Online 3B lower merge
TS13: sinus venosus Absent Online 3B lower merge , Online 3B lower inset , Online 3B lower green
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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