Probe:	Mm-Nmb (459931)
Probe preparation:	Antisense RNA
Visualized with:	Fluorescein
Assay notes:	RNAscope assay.

Structure	Level	Pattern	Image	Note
TS28: midbrain tegmentum	Present	Single cells	3A

Structure	Level	Pattern	Image	Note
TS28: nucleus incertus	Present	Regionally restricted	3B , 3B merge , 3B red	Expressing neurons are seen in the rostral nucleus incertus.

Structure	Level	Pattern	Image	Note
TS28: nucleus incertus	Present	Regionally restricted	3C , 3C merge , 3C red	Expressing neurons are seen in the central nucleus incertus.

Structure	Level	Pattern	Image	Note
TS28: nucleus incertus	Present	Single cells	3D merge , 3D , 3D red	Expressing neurons are seen in the caudal nucleus incertus.
TS28: brain gray matter	Present	Regionally restricted	3D	Expressing neurons are seen in the central pontine gray.

Structure	Level	Pattern	Image	Note
TS28: brain gray matter	Present	Single cells	3E , 3E red , 3E merge	Expressing neurons are seen in the central pontine gray.

Structure	Level	Pattern	Image	Note
TS28: dorsal tegmental nucleus	Present	Single cells	3F	Expressing neurons are seen in the posterodorsal tegmental nucleus.

Structure	Level	Pattern	Image	Note
TS28: nucleus incertus	Present	Regionally restricted	4A merge , 4A green , 4A	Expressing neurons are seen in the rostral nucleus incertus.

Structure	Level	Pattern	Image	Note
TS28: nucleus incertus	Present	Regionally restricted	4B green , 4B merge , 4B	Expressing neurons are seen in the central nucleus incertus.

Structure	Level	Pattern	Image	Note
TS28: nucleus incertus	Present	Regionally restricted	4C1 merge , 4C , 4C1 green	Expressing neurons are seen in the caudal nucleus incertus.
TS28: brain gray matter	Present	Regionally restricted	4C , 4C2 merge , 4C2 green	Expressing neurons are seen in the central pontine gray.

Structure	Level	Pattern	Image	Note
TS28: nucleus incertus	Present	Regionally restricted	4D merge , 4D , 4D green	Expressing neurons are seen in the rostral nucleus incertus.

Structure	Level	Pattern	Image	Note
TS28: nucleus incertus	Present	Regionally restricted	4E , 4E merge , 4E green	Expressing neurons are seen in the central nucleus incertus.

Structure	Level	Pattern	Image	Note
TS28: nucleus incertus	Present	Regionally restricted	4F , 4F1 green , 4F1 merge	Expressing neurons are seen in the caudal nucleus incertus.
TS28: brain gray matter	Present	Regionally restricted	4F2 merge , 4F , 4F2 green	Expressing neurons are seen in the central pontine gray.