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Gene Expression Data
Assay Details
Assay
Reference: J:302432 Nasirova N, et al., Mapping Cell Types and Efferent Pathways in the Ascending Relaxin-3 System of the Nucleus Incertus. eNeuro. 2020 Nov/Dec;7(6):ENEURO.0272-20.2020
Assay type: RNA in situ
MGI Accession ID: MGI:6719374
Gene symbol: Gad2
Gene name: glutamic acid decarboxylase 2
Probe: Mm-Gad2 (439371)
Probe preparation: Antisense RNA
Visualized with: Fluorescein
Assay notes: RNAscope assay.
Results
Specimen 1K: postnatal (more )
Note: Double labeled: red - Gad2; green - Rln3 (assay MGI:6719268)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Regionally restricted 1K3 , 1K2 GABAergic neurons are seen in the nucleus incertus.
TS28: brain gray matter Present Regionally restricted 1K3 , 1K2 GABAergic neurons are widespread in the central pontine gray.

Specimen 4A: postnatal (more )
Note: Double labeled: red - Gad2; green - Nmb (assay MGI:6719378)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Regionally restricted 4A red , 4A , 4A merge GABAergic neurons are seen in the rostral nucleus incertus.
TS28: dorsal tegmental nucleus Present 4A
TS28: laterodorsal tegmental nucleus Present 4A

Specimen 4B: postnatal (more )
Note: Double labeled: red - Gad2; green - Nmb (assay MGI:6719378)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Regionally restricted 4B merge , 4B , 4B red GABAergic neurons are seen in the central nucleus incertus.
TS28: brain gray matter Present Regionally restricted 4B GABAergic neurons are seen in the central pontine gray.
TS28: dorsal tegmental nucleus Present 4B

Specimen 4C: postnatal (more )
Note: Double labeled: red - Gad2; green - Nmb (assay MGI:6719378)
Structure Level Pattern Image Note
TS28: nucleus incertus Present Regionally restricted 4C , 4C1 red , 4C1 merge GABAergic neurons are seen in the caudal nucleus incertus.
TS28: brain gray matter Present Regionally restricted 4C2 red , 4C , 4C2 merge GABAergic neurons are seen in the central pontine gray.
TS28: dorsal tegmental nucleus Present 4C GABAergic neurons are seen in the posterodorsal tegmental nucleus.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory