Gene Expression Data
Assay Details
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Assay
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Reference:
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J:302432
Nasirova N, et al., Mapping Cell Types and Efferent Pathways in the Ascending Relaxin-3 System of the Nucleus Incertus. eNeuro. 2020 Nov/Dec;7(6):ENEURO.0272-20.2020
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6719268
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Gene symbol:
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Rln3
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Gene name:
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relaxin 3
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Probe:
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Mm-Rln3 (459921)
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Probe preparation:
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Antisense RNA
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Visualized with:
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Fluorescein
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Assay notes:
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RNAscope assay.
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Results
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Specimen
1D:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Rln3tm1.1(cre/GFP)Et/Rln3+
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rln3 RNA; green - Rln3 reporter (assay MGI:6718943)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain gray matter |
Present |
Regionally restricted |
1D
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Expression is seen in a small fraction of neurons in the central pontine gray.
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Specimen
1F:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Rln3tm1.1(cre/GFP)Et/Rln3+
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rln3 RNA; green - Rln3 reporter (assay MGI:6718943)
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Specimen
1H:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Rln3tm1.1(cre/GFP)Et/Rln3+
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rln3 RNA; green - Rln3 reporter (assay MGI:6718943)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: nucleus incertus |
Present |
Regionally restricted |
1H
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Expressing neurons are seen in the caudal nucleus incertus.
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Specimen
1K:
postnatal
(more  )
Note: Double labeled: green - Rln3; red - Gad2 (assay MGI:6719374)
Specimen
1K:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rln3; red - Gad2 (assay MGI:6719374)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: nucleus incertus |
Present |
Regionally restricted |
1K3
,
1K1
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Expressing neurons are seen in the nucleus incertus.
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| TS28: brain gray matter |
Present |
Regionally restricted |
1K1
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1K3
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Expression is seen in a small fraction of neurons in the central pontine gray.
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Specimen
3A:
postnatal
(more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
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Specimen
3B:
postnatal
(more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
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Specimen
3C:
postnatal
(more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
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Specimen
3D:
postnatal
(more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Specimen
3D:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
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Specimen
3E:
postnatal
(more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
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Specimen
3F:
postnatal
(more )
Note: Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
Specimen
3F:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rln3; red - Nmb (assay MGI:6719378)
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Analysis Tools