Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:207611 Lange AW, et al., Sox17 is required for normal pulmonary vascular morphogenesis. Dev Biol. 2014 Mar 1;387(1):109-20
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6717427
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	anti-alpha-SMA
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

6D ©

Specimen 6D: postnatal week 2 (more

)
Note: Double labeled: green - Acta2; red - Emcn (assay MGI:6717450).

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present	Regionally restricted	6D	Expression was observed surrounding larger pulmonary blood vessels.

6E ©

Specimen 6E: postnatal week 2; Sox17^tm1Jaw/Sox17^tm1Jaw, Twist2^{tm1.1(cre)Dor}/Twist2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Acta2; red - Emcn (assay MGI:6717450).

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present	Regionally restricted	6E	Expression was observed surrounding larger pulmonary blood vessels.

6F ©

Specimen 6F: postnatal week 2; Sox17^tm1Jaw/Sox17^tm1Jaw, Twist2^{tm1.1(cre)Dor}/Twist2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Acta2; red - Emcn (assay MGI:6717450).

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present	Regionally restricted	6F	Expression was observed surrounding larger pulmonary blood vessels.

6G ©

Specimen 6G: postnatal week 2 (more

)
Note: Double labeled: green - Acta2; red - Emcn (assay MGI:6717450).

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present	Regionally restricted	6G	Expression was observed surrounding larger pulmonary blood vessels.

6H ©

Specimen 6H: postnatal week 2; Sox17^tm1Jaw/Sox17^tm1Jaw, Twist2^{tm1.1(cre)Dor}/Twist2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Acta2; red - Emcn (assay MGI:6717450).

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present	Regionally restricted	6H	Expression was observed surrounding larger pulmonary blood vessels.

6I ©

Specimen 6I: postnatal week 2; Sox17^tm1Jaw/Sox17^tm1Jaw, Twist2^{tm1.1(cre)Dor}/Twist2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Acta2; red - Emcn (assay MGI:6717450).

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present	Regionally restricted	6I	Expression was observed surrounding larger pulmonary blood vessels.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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