Probe:	Lrrk2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors did not specify which probe was used, Lrrk2 cDNA3 (MGI:6705037) may have been used instead.

Structure	Level	Pattern	Image	Note
TS24: neocortex	Present		1A

Structure	Level	Pattern	Image	Note
TS17: hindbrain floor plate	Present		2A
TS17: optic cup outer layer	Present		2A
TS17: head mesenchyme	Present	Regionally restricted	2A	Expression is surrounding the optic cup.
TS17: optic cup inner layer	Absent		2A
TS17: telencephalon	Absent		2A

Structure	Level	Pattern	Image	Note
TS17: optic cup outer layer	Present		2B
TS17: head mesenchyme	Present	Regionally restricted	2B	Expression is surrounding the optic cup.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak	Scattered	2C	Expression is in scattered cells of the intermediate portion of the neural tube.
TS17: urogenital ridge	Present		2C

Structure	Level	Pattern	Image	Note
TS20: basal columns	Moderate		2D

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Regionally restricted	2E	Expression is throughout except in the superficial layer.
TS22: telencephalon	Strong	Regionally restricted	2E	Expression is throughout except in the superficial layer.

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle ependyma	Present		2F
TS22: cerebellum marginal layer	Present		2F

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Strong		2G
TS24: cerebral cortex ventricular layer	Strong		2G
TS24: cortical plate	Strong		2G
TS24: cerebral cortex intermediate zone	Absent		2G
TS24: cortical subplate	Absent		2G
TS24: ganglionic eminence	Absent		2G

Structure	Level	Pattern	Image	Note
TS24: brainstem nucleus	Moderate	Regionally restricted	2H	Expression is in the pontine nuclei and nucleus raphe magnus.
TS24: metencephalon roof	Moderate		2H

Structure	Level	Pattern	Image	Note
TS24: spinal cord gray matter	Present		2I
TS24: dorsal grey horn	Strong		2I	Expression levels are highest in layers III and IV of the dorsal horn.

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		2J
TS27: neocortex	Weak		2J

Structure	Level	Pattern	Image	Note
TS27: CA1 pyramidal cell layer	Strong		2K
TS27: dentate gyrus granule cell layer	Strong		2K
TS27: hippocampus CA2	Strong	Regionally restricted	2K	Expression is in pyramidal neurons.
TS27: hippocampus CA3	Strong	Regionally restricted	2K	Expression is in pyramidal neurons.
TS27: hippocampus	Strong	Regionally restricted	2K	Expression is in the area of the alveus.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Moderate		2L
TS27: cerebellum external granule cell layer	Moderate		2L
TS27: cerebellum internal granule cell layer	Moderate		2L

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		3A

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3B

Structure	Level	Pattern	Image	Note
TS28: basolateral amygdaloid nucleus	Present		3C

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present		3D

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars compacta	Present		3E

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		3F

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present		5A
TS13: 2nd branchial arch	Present		5A
TS13: unsegmented mesenchyme	Present		5A
TS13: trunk somite	Present	Regionally restricted	5A	Expression is in the first somites within the caudal region of the trunk.
TS13: neural ectoderm	Absent		5A

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		5B
TS17: 2nd branchial arch	Present		5B
TS17: eye	Present		5B
TS17: forelimb bud apical ectodermal ridge	Present		5B
TS17: hindbrain floor plate	Present		5B
TS17: hindlimb bud apical ectodermal ridge	Present		5B
TS17: nasal process	Present	Regionally restricted	5B	Expression is in the outer cell layers.
TS17: bulbus cordis	Absent		5B
TS17: midbrain	Absent		5B
TS17: telencephalon	Absent		5B

Structure	Level	Pattern	Image	Note
TS22: forelimb interdigital region	Present		5C
TS22: forelimb digit	Absent		5C

Structure	Level	Pattern	Image	Note
TS20: somite	Present		5D

Structure	Level	Pattern	Image	Note
TS22: limb interdigital region	Present		5E
TS22: limb digit	Absent		5E

Structure	Level	Pattern	Image	Note
TS24: vomeronasal organ	Present		5F

Structure	Level	Pattern	Image	Note
TS22: nephron	Very strong	Regionally restricted	5G	Expression is in presumptive podocytes of the developing nephrons.

Structure	Level	Pattern	Image	Note
TS24: nephron	Very strong	Regionally restricted	5H	Expression is in presumptive podocytes of the developing nephrons.
TS24: comma-shaped body	Present		5H

Structure	Level	Pattern	Image	Note
TS27: nephron	Very strong	Regionally restricted	5I	Expression is in presumptive podocytes of the developing nephrons.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Very strong		5J

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	5K	Expression is in a subpopulation of large neurons.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium	Present		5L

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present		5M

Structure	Level	Pattern	Image	Note
TS24: alveolus epithelium	Present		5N

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present		S1A

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex ventricular zone	Present		S1C

Structure	Level	Pattern	Image	Note
TS24: lung	Present		S1E

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present		S1G

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		S1I

Structure	Level	Pattern	Image	Note
TS27: hindbrain	Present		S1K
TS27: raphe nucleus	Present		S1K

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		S1M

Structure	Level	Pattern	Image	Note
TS28: testis	Strong		S1O

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted		Expression is in the distal parts.
TS17: hindlimb bud	Present	Regionally restricted		Expression is in the distal parts.
TS17: hindbrain ventricular layer	Present
TS17: neural tube	Weak	Regionally restricted		Expression is in the intermediate portion.
TS17: dorsal root ganglion	Absent

Structure	Level	Pattern	Image	Note
TS20: foot	Present	Regionally restricted		Expression is in the distal parts.
TS20: hand	Present	Regionally restricted		Expression is in the distal parts.
TS20: cerebral cortex	Present			Expression is in all layers.
TS20: spinal cord floor plate	Present
TS20: hindbrain ventricular layer	Moderate
TS20: telencephalon	Moderate
TS20: dorsal root ganglion	Absent
TS20: metanephros	Absent

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present
TS22: cerebral cortex subventricular zone	Present
TS22: cerebral cortex ventricular layer	Present
TS22: cortical plate	Present
TS22: dorsal root ganglion	Present
TS22: limb interdigital region	Present
TS22: spinal cord gray matter	Present
TS22: visceral epithelium of capillary loop renal corpuscle	Present
TS22: spinal cord dorsal horn	Strong
TS22: brainstem nucleus	Present	Regionally restricted		Expression is in the pontine nucleus.

Structure	Level	Pattern	Image	Note
TS24: spinal cord dorsal horn	Strong	Regionally restricted		Expression is in layer III/IV of the dorsal horn.
TS24: 4th ventricle ependyma	Present
TS24: cerebellum	Present
TS24: cerebral cortex subventricular zone	Present
TS24: cerebral cortex ventricular layer	Present
TS24: cortical plate	Present
TS24: dorsal root ganglion	Present
TS24: limb interdigital region	Present
TS24: spinal cord gray matter	Present
TS24: visceral epithelium of capillary loop renal corpuscle	Present

Structure	Level	Pattern	Image	Note
TS26: spinal cord dorsal horn	Strong			Expression is in layer III/IV of the dorsal horn.
TS26: cerebellum external granule cell layer	Present
TS26: cerebral cortex subventricular zone	Present
TS26: cortical plate	Present
TS26: dorsal root ganglion	Present
TS26: visceral epithelium of capillary loop renal corpuscle	Present
TS26: cerebellum granule cell layer	Present			Expression is in the internal and external granule layer.
TS26: cerebral cortex ventricular zone	Weak
TS26: foot	Absent
TS26: hand	Absent

Structure	Level	Pattern	Image	Note
TS27: spinal cord dorsal horn	Strong			Expression is in layer III/IV of the dorsal horn.
TS27: cerebellum external granule cell layer	Present
TS27: cerebellum internal granule cell layer	Present
TS27: cortical layer II	Present
TS27: dorsal root ganglion	Present
TS27: pontine nucleus	Present
TS27: spinal cord gray matter	Present
TS27: visceral epithelium of capillary loop renal corpuscle	Present
TS27: cortical layer I	Weak
TS27: cortical layer III	Weak
TS27: cortical layer IV	Weak
TS27: cortical layer V	Weak
TS27: cortical layer VI	Weak
TS27: foot	Absent
TS27: hand	Absent

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Strong
TS28: cerebellum Purkinje cell layer	Strong
TS28: olfactory tubercle	Strong
TS28: anterior cortical amygdaloid nucleus	Moderate
TS28: basolateral amygdaloid nucleus	Moderate
TS28: cochlear nucleus	Moderate
TS28: cortical amygdaloid nucleus	Moderate
TS28: dentate gyrus granule cell layer	Moderate
TS28: islands of Calleja	Moderate
TS28: piriform cortex	Moderate
TS28: trigeminal V mesencephalic nucleus	Moderate
TS28: auditory cortex	Moderate	Regionally restricted		Expression is in layers II, III, and V.
TS28: primary visual cortex	Moderate	Regionally restricted		Expression is in layers II, III, and V.
TS28: cingulate cortex	Moderate	Regionally restricted		Expression is in layers II and III.
TS28: frontal cortex	Moderate	Regionally restricted		Expression is in layers II and III.
TS28: retrosplenial cortex	Moderate	Regionally restricted		Expression is in layers II and III.
TS28: cerebral cortex	Moderate			Expression is in all layers of the motor cortex.
TS28: primary somatosensory cortex	Moderate	Regionally restricted		Expression is in layers IV and V.
TS28: secondary somatosensory cortex	Moderate	Regionally restricted		Expression is in layer IV.
TS28: CA1 pyramidal cell layer	Weak
TS28: CA3 pyramidal cell layer	Weak
TS28: accumbens nucleus	Weak
TS28: cerebellum granule cell layer	Weak
TS28: main olfactory bulb glomerular layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: main olfactory bulb mitral cell layer	Weak
TS28: substantia nigra pars compacta	Weak
TS28: ventral tegmental area	Weak
TS28: entorhinal cortex	Weak	Regionally restricted		Expression is in layer II.
TS28: globus pallidus	Absent
TS28: substantia nigra pars reticulata	Absent

Structure	Level	Pattern	Image	Note
TS28: renal proximal convoluted tubule	Weak	Regionally restricted		Expression is in the S3 section.
TS28: trigeminal ganglion	Weak	Regionally restricted		Expression is in a subpopulation of neurons.