Sox2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:278249 Iwafuchi-Doi M, et al., The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates. Dev Biol. 2011 Apr 15;352(2):354-66
Assay type:	RNA in situ
MGI Accession ID:	MGI:6682144
Gene symbol:	Sox2
Gene name:	SRY (sex determining region Y)-box 2

Probe:

Sox2 probe25

Results

1A(a) E6.5 ©

Specimen 1Aa E6.5: embryonic day 6.5 (more

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Structure	Level	Pattern	Image	Note
TS8: epiblast	Present		1A(a) E6.5

1A(a) E7.75 ©

Specimen 1Aa E7.75: embryonic day 7.75 (more

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Structure	Level	Pattern	Image	Note
TS11: neural plate	Present	Regionally restricted	1A(a) E7.75	Expression was detected in anterior neural plate.

1A(a) E8.5 ©

Specimen 1Aa E8.5: embryonic day 8.5 (more

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Structure	Level	Pattern	Image	Note
TS13: embryo	Present		1A(a) E8.5

1C(a) N2+/+ E6.75 ©

Specimen 1Ca N2+/+ E6.75: embryonic day 6.75 (more

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Structure	Level	Pattern	Image	Note
TS9: extraembryonic component	Present	Regionally restricted	1C(a) N2+/+ E6.75	Expression was detected in the periphery of the chorion.
TS9: embryo	Present	Regionally restricted	1C(a) N2+/+ E6.75	Expression was detected in the epiblast.

1C(a) N2-/- E6.75 ©

Specimen 1Ca N2-/- E6.75: embryonic day 6.75; Rr550^tm1.1Knd/Rr550^tm1.1Knd (more

)

Structure	Level	Pattern	Image	Note
TS9: extraembryonic component	Present	Regionally restricted	1C(a) N2-/- E6.75	Sox2 expression in the periphery of the chorion was unaffected in the N2-/- homozygous embryo.
TS9: embryo	Weak	Regionally restricted	1C(a) N2-/- E6.75	Expression was greatly reduced in the epiblast.

1C(a) N2+/+ E7.5 ©

Specimen 1Ca N2+/+ E7.5: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: chorion	Present	Regionally restricted	1C(a) N2+/+ E7.5	Expression was detected in the periphery of the chorion.
TS11: neural plate	Present	Regionally restricted	1C(a) N2+/+ E7.5	Expression was detected in the anterior neural plate.

1C(a) N2-/- E7.5 ©

Specimen 1Ca N2-/- E7.5: embryonic day 7.5; Rr550^tm1.1Knd/Rr550^tm1.1Knd (more

)

Structure	Level	Pattern	Image	Note
TS11: chorion	Present	Regionally restricted	1C(a) N2-/- E7.5	Sox2 expression in the periphery of the chorion was unaffected in the N2-/- homozygous embryo.
TS11: neural plate	Weak	Regionally restricted	1C(a) N2-/- E7.5	Expression was greatly reduced in the anterior neural plate.

1C(a) N2+/+ E7.75 ©

Specimen 1Ca N2+/+ E7.75: embryonic day 7.75 (more

)

Structure	Level	Pattern	Image	Note
TS11: chorion	Present	Regionally restricted	1C(a) N2+/+ E7.75	Expression was detected in the periphery of the chorion.
TS11: neural plate	Present	Regionally restricted	1C(a) N2+/+ E7.75	Expression was detected in the anterior neural plate.
TS11: embryo	Present	Regionally restricted	1C(a) N2+/+ E7.75	Expression was detected in the posterior half of the embryo.

1C(a) N2-/- E7.75 ©

Specimen 1Ca N2-/- E7.75: embryonic day 7.75; Rr550^tm1.1Knd/Rr550^tm1.1Knd (more

)

Structure	Level	Pattern	Image	Note
TS11: chorion	Present	Regionally restricted	1C(a) N2-/- E7.75	Sox2 expression in the periphery of the chorion was unaffected in the N2-/- homozygous embryo.
TS11: neural plate	Weak	Regionally restricted	1C(a) N2-/- E7.75	Expression was greatly reduced in the anterior neural plate.
TS11: embryo	Present	Regionally restricted	1C(a) N2-/- E7.75	Sox2 expression in the posterior half of the embryos initiated in the N2-/- embryo.

S2A(a) N2+/+ E8.25 left ©

Specimen S2A(a) N2+/+ left: embryonic day 8.25 (more

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Note: 5-6 somites.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		S2A(a) N2+/+ E8.25 left	Expression was detected in the forebrain.

Specimen S2A(a) N2-/- left: embryonic day 8.25; Rr550^tm1.1Knd/Rr550^tm1.1Knd (more

)
Note: 5-6 somites.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	S2A(a) N2-/- E8.25 left	Expression was initiated in the N2-/- forebrain, but the expression level was lower than in wild type.

Specimen S2A(a) N2+/+ right: embryonic day 8.25 (more

)
Note: 5-6 somites.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	S2A(a) N2+/+ E8.25 right	Expression was detected in the forebrain.

Specimen S2A(a) N2-/- right: embryonic day 8.25; Rr550^tm1.1Knd/Rr550^tm1.1Knd (more

)
Note: 5-6 somites.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	S2A(a) N2-/- E8.25 right	Expression was initiated in the N2-/- forebrain, but the expression level was lower than in wild type.

Specimen S2A(b) N2+/+: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present		S2A(b) N2+/+ E10.5

Specimen S2A(b) N2-/-: embryonic day 10.5; Rr550^tm1.1Knd/Rr550^tm1.1Knd (more

)

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present		S2A(b) N2-/- E10.5	Sox2 expression at E10.5 is indistinguishable between wild type and N2-/- embryos.

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