Reporter:	GFP
	Direct Detection
Assay notes:	Fixative was formalin or 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS14: embryo	Present

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		1A/B
TS15: neural tube	Present		1A/B
TS15: tail	Present	Regionally restricted	1A/B	Expression is at the tip of the tail.

Structure	Level	Pattern	Image	Note
TS17: tail	Strong		1D
TS17: forelimb bud	Strong	Regionally restricted	1D	Expression is at the distal end.
TS17: hindlimb bud	Strong	Regionally restricted	1D	Expression is at the distal end.
TS17: neural tube	Present		1D
TS17: embryo mesoderm	Present	Regionally restricted	1D	Expression is in the flank mesoderm from the mid-hindlimb region to the posterior tip of the tail.

Structure	Level	Pattern	Image	Note
TS19: limb	Present	Regionally restricted	1E/F	Expression is in a more proximal region.
TS19: tail	Present		1E/F
TS19: autopod	Weak		1E/F

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted	1G/H	Expression is in a more proximal region.
TS20: tail	Present		1G/H
TS20: autopod	Weak		1G/H

Structure	Level	Pattern	Image	Note
TS21: limb	Present	Regionally restricted	1J	Expression is in a more proximal region.
TS21: autopod	Weak		1J
TS21: tail	Weak		1J

Structure	Level	Pattern	Image	Note
TS23: limb	Present	Regionally restricted	1K/L	Expression is in a more proximal region.
TS23: spinal cord	Present		1K/L
TS23: autopod	Weak		1K/L
TS23: tail	Weak		1K/L

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	2A	Expression is uniform in the distal limb bud and in a subset of cells proximally.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	2B	Expression is uniform in the distal limb bud and in a subset of cells proximally and is absent in the center of the proximal limb.

Structure	Level	Pattern	Image	Note
TS20: zeugopod	Present		2C
TS20: autopod	Weak		2C
TS20: forelimb interdigital region mesenchyme	Weak		2C

Structure	Level	Pattern	Image	Note
TS21: forelimb mesenchyme	Present	Regionally restricted	2E	Expression is distal surrounding the distal radius and ulna in perichondrial regions.
TS21: zeugopod	Present	Regionally restricted	2E	Expression is distal surrounding the distal radius and ulna in perichondrial regions.
TS21: radius-ulna cartilage condensation	Present	Regionally restricted	2E	Expression is at the distal ends of the developing radius and ulna.
TS21: forelimb interdigital region	Weak		2E

Structure	Level	Pattern	Image	Note
TS23: forelimb mesenchyme	Present	Regionally restricted	2F	Expression is distal surrounding the distal radius and ulna in perichondrial regions.
TS23: zeugopod	Present	Regionally restricted	2F	Expression is distal surrounding the distal radius and ulna in perichondrial regions.
TS23: hand mesenchyme	Present	Regionally restricted	2F	Expression is in cells surrounding the condensing digit cartilage.

Structure	Level	Pattern	Image	Note
TS17: metanephric mesenchyme	Present	Regionally restricted	3A	Expression is in the condensing metanephric mesenchyme.
TS17: ureteric bud	Absent		3A

Structure	Level	Pattern	Image	Note
TS19: metanephric mesenchyme	Present	Regionally restricted	3B	Expression is in the condensing metanephric mesenchyme.
TS19: ureteric bud	Absent		3B

Structure	Level	Pattern	Image	Note
TS20: mesenchyme of metanephros	Present	Regionally restricted	3C	Expression is in the condensing nephrogenic mesenchyme surrounding the ureteric bud.

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present	Regionally restricted	3F , 3D	Expression is in the condensing nephrogenic mesenchyme surrounding the ureteric bud and surrounding the ureter.
TS21: ureteric tip	Absent		3D , 3F

Structure	Level	Pattern	Image	Note
TS23: mesenchyme of metanephros	Present		3G	Expression is in the condensing nephrogenic mesenchyme cells and in mesenchyme surrounding the ureteric bud.
TS23: ureteric tip	Absent		3G

Structure	Level	Pattern	Image	Note
TS24: early nephron	Present	Regionally restricted	3H	Expression is in the nephrogenic progenitor cells.
TS24: cortical renal tubule	Present	Regionally restricted	3H	There is some expression in convoluted tubules.

Structure	Level	Pattern	Image	Note
TS24: uterus	Present		4A
TS24: ovary	Absent		4A

Structure	Level	Pattern	Image	Note
TS24: ductus deferens	Very strong		4B

Structure	Level	Pattern	Image	Note
TS27: uterus	Strong		4C
TS27: ovary	Absent		4C

Structure	Level	Pattern	Image	Note
TS27: ductus deferens	Very strong		4D

Structure	Level	Pattern	Image	Note
TS28: uterus	Strong		4E
TS28: ovary	Absent		4E

Structure	Level	Pattern	Image	Note
TS28: ductus deferens	Very strong		4F

Structure	Level	Pattern	Image	Note
TS28: uterus	Strong		4G
TS28: ovary	Absent		4G

Structure	Level	Pattern	Image	Note
TS28: ductus deferens	Very strong		4H

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present		5A

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	5B	Expression is in dorsal and medial regions.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	5C	Expression is in dorsal and medial regions.

Structure	Level	Pattern	Image	Note
TS27: limb	Present
TS27: spinal cord	Present
TS27: tail	Present

Structure	Level	Pattern	Image	Note
TS28: early nephron	Present	Regionally restricted		Expression in the nephrogenic progenitor cells is maintained through P8 and is absent after this stage.

Structure	Level	Pattern	Image	Note
TS28: early nephron	Absent			Expression in the nephrogenic progenitor cells is maintained through P8 and is absent after this stage.

Structure	Level	Pattern	Image	Note
TS28: uterus	Strong

Structure	Level	Pattern	Image	Note
TS28: ductus deferens	Strong