Gene Expression Data
Assay Details
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Assay
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Reference:
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J:98918
Kassar-Duchossoy L, et al., Pax3/Pax7 mark a novel population of primitive myogenic cells during development. Genes Dev. 2005 Jun 15;19(12):1426-31
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6489363
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Gene symbol:
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Pax7
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Gene name:
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paired box 7
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Results
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Specimen
1A Pax7:
embryonic day 9.75
(more  )
Specimen
1A Pax7:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: dermomyotome |
Present |
Regionally restricted |
1A Pax7
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Coexpression with Pax7 in almost all dermomyotomal cells. Preferentially expressed in central dermomyotome.
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| TS16: myotome |
Ambiguous |
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1A Pax7
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Majority of myotomal cells are Pax7-negative before E10.5
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Specimen
1D:
embryonic day 11.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: dermomyotome |
Present |
Regionally restricted |
1D
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Pax7-expressing cells released from central portion of demomyotome.
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| TS19: myotome |
Present |
Regionally restricted |
1D
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Expression in cells entering myotome on dorsal side.
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Specimen
1D Pax7:
embryonic day 11.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
Specimen
1D Pax7:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Regionally restricted |
1D Merge
,
1D Pax7
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Expressing cells become dispersed among differentiating myotomal cells.
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Specimen
1F:
embryonic day 11.5; Pax3Sp/ Pax3Sp
(more )
Note: Double labeled: red - Pax7; green - MyHC (myosin heavy chain).
Specimen
1F:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - MyHC (myosin heavy chain).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Regionally restricted |
1F
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Authors report exclusion with MyHc in myotome.
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Specimen
1F Pax7:
embryonic day 11.5; Pax3Sp/ Pax3Sp
(more )
Note: Double labeled: red - Pax7; green - MyHC (myosin heavy chain).
Specimen
1F Pax7:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Pax7; green - MyHC (myosin heavy chain).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Regionally restricted |
1F Merge
,
1F Pax7
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Authors report exclusion with MyHc in myotome.
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Specimen
1G Pax7 (upper):
embryonic day 11.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: red - Pax7; green - Des (assay MGI:6489365).
Specimen
1G Pax7 (upper):
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Double labeled: red - Pax7; green - Des (assay MGI:6489365).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Regionally restricted |
1G Merge (upper)
,
1G Pax7 (upper)
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Pax7-expressing cells dispersed amond differentiating myotomal cells. Majority of expressing cells excluded from myoblasts.
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Specimen
1G Pax7 (lower):
embryonic day 11.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: red - Pax7; green - Myog (assay MGI:6489366).
Specimen
1G Pax7 (lower):
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Double labeled: red - Pax7; green - Myog (assay MGI:6489366).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Regionally restricted |
1G Merge (lower)
,
1G Pax7 (lower)
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Pax7-expressing cells dispersed amond differentiating myotomal cells. Majority of expressing cells excluded from differentiating cells.
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Specimen
2D Pax7:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Myf5tm2Tajb/Myf5tm2Tajb
Myf6tm1Tajb/Myf6tm1Tajb
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; dark nuclei - Myf6 reporter (assay MGI:6489375).
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Specimen
3A Pax7:
embryonic day 14.0; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
Specimen
3A Pax7:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S4/SvJae
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Mutant Allele(s):
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Myf5tm1Tajb/Myf5tm1Tajb
Myod1tm1Jae/Myod1tm1Jae
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Pax7; green - Des (assay MGI:6489365); blue - Hoechst.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: intercostal muscle |
Present |
Regionally restricted |
3B
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Authors report expression in cells associated with residual intercostal embryonic fibers in absence of myogenesis.
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Specimen
3C Pax7:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S4/SvJae
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Mutant Allele(s):
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Myf5tm1Tajb/Myf5tm1Tajb
Myod1tm1Jae/Myod1tm1Jae
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489374).
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Specimen
3D Pax7 (right):
embryonic day 14.5; Myf5tm1Tajb/Myf5 +
(more )
Note: Double labeled: red - Pax7; green - Casp3 (assay MGI:6489368).
Specimen
3D Pax7 (right):
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Myf5tm1Tajb/Myf5+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - Casp3 (assay MGI:6489368).
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Specimen
4A Pax7:
embryonic day 16.5
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
4A Pax7:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - laminin.
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Specimen
4B Pax7:
embryonic day 18.5; Myf5tm1Pas/Myf5 +
(more )
Note: Triple labeled: red - Pax7; green - laminin; green - PM-histone H3.
Specimen
4B Pax7:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Pax7; green - laminin; green - PM-histone H3.
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Specimen
4C Pax7:
postnatal newborn; Myf5tm1Pas/Myf5 +
(more )
Specimen
4C Pax7:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: forelimb muscle |
Present |
Regionally restricted |
4C Pax7
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Expression in satellite cells from freshly isolated forelimb fiber.
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Specimen
4D Pax7:
(close )
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Genetic Background:
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involves: C57BL
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
Pax3Sp/Pax3Sp
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; dark nuclei - Myf5 reporter (assay MGI:6489371).
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Specimen
S1A Pax7 (left):
embryonic day 11.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
Specimen
S1A Pax7 (left):
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
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Specimen
S1A Pax7 (right):
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5tm1Pas
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - Myf5 reporter (assay MGI:6489373).
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Specimen
S1B Pax7:
embryonic day 11.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: red - Pax7; green - Pax3 (assay MGI:6489364).
Specimen
S1B Pax7:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Pax7; green - Pax3 (assay MGI:6489364).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Regionally restricted |
S1B Pax7
,
S1B Merge
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Coexpression with Pax3 in cells released from central dermomyotome into myotome.
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Specimen
S1D Pax7:
embryonic day 11.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: red - Pax7; green - PM-H3.
Specimen
S1D Pax7:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - PM-H3.
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Specimen
S2 Pax7:
embryonic day 11.5; Myf5tm1Pas/Myf5 +
(more )
Note: Double labeled: red - Pax7; dark nuclei - Myf5 reporter (assay MGI:6489371).
Specimen
S2 Pax7:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Myf5tm1Pas/Myf5+
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Age:
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embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
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Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Pax7; dark nuclei - Myf5 reporter (assay MGI:6489371).
|
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S4/SvJae
|
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Mutant Allele(s):
|
Myf5tm1Tajb/Myf5tm1Tajb
Myod1tm1Jae/Myod1tm1Jae
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Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
|
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
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Embedding:
|
Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: skeletal musculature |
Present |
Regionally restricted |
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Expression in clusters in presumed muscle forming regions.
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Analysis Tools