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Gene Expression Data
Assay Details
Assay
Reference: J:245763 Gao L, et al., Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017 Oct 01;144(19):3511-3520
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6467789
Gene symbol: Pard6b
Gene name: par-6 family cell polarity regulator beta
Antibody: anti-PARD6B (H-64; sc-67392)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1A f/f 4um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-1 Expressed at the apical surface, which showed a normal continuous lumen.
Specimen 1A f/f 11um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-2 Expressed at the apical surface, which showed a normal continuous lumen.
Specimen 1A f/f 20um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-3 Expressed at the apical surface, which showed a normal continuous lumen.
Specimen 1A f/f 3D: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A f/f-4 Expressed at the apical surface, which showed a normal continuous lumen.
Specimen 1A cKO 4um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-1 Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen 1A cKO 11um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-2 Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen 1A cKO 20um: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-3 Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen 1A cKO 3D: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1A cKO-4 Expressed at the apical surface, which showed a partially absent and numerous discontinuous lumen.
Specimen 1C: embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: blue - Pard6b; red - Ncam1 (assay MGI:6467813).
Structure Level Pattern Image Note
TS25: s-shaped body Present 1C Expressed at the apical surface in a slightly later, more elongated tubule, which showed a partially absent and numerous discontinuous lumen. And more than one lumen transversely, appearing multi-layered.
Specimen 5A/A': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: blue - Pard6b; red - alpha-tubulin; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS25: renal vesicle Present 5A' , 5A Expressed at the apical surface, which showed a normal continuous lumen. The orientation of division appeared to be parallel to the luminal/apical surface.
Specimen 5B/B': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: blue - Pard6b; red - alpha-tubulin; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS25: renal vesicle Present 5B' , 5B Expressed at the apical surface, which showed a normal continuous lumen. The orientation of division appeared to be parallel to the luminal/apical surface.
Specimen 5C/C': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: blue - Pard6b; red - alpha-tubulin; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS25: renal vesicle Present 5C , 5C' Expressed at the apical surface, which showed a normal continuous lumen. The midbody was formed apically.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory