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Gene Expression Data
Assay Details
Assay
Reference: J:262744 Shetty R, et al., Rudhira/BCAS3 is essential for mouse development and cardiovascular patterning. Sci Rep. 2018 Apr 4;8(1):5632
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6456236
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1h TS Control: embryonic day 9.5 (more )
Note: Double labeled: green - Acta2; red - Ly76 (assay MGI:6456209).
Structure Level Pattern Image Note
TS15: atrium cardiac muscle Present 1h upper left
TS15: primitive ventricle cardiac muscle Present 1h upper left
Specimen 1h TS -/-: embryonic day 9.5; Bcas3tm1.1Msin/Bcas3tm1.1Msin (more )
Note: Double labeled: green - Acta2; red - Ly76 (assay MGI:6456209).
Structure Level Pattern Image Note
TS15: atrium cardiac muscle Present 1h upper right Collapsed, smaller heart chambers and a fused atrio-ventricular canal were seen.
TS15: primitive ventricle cardiac muscle Present 1h upper right Collapsed, smaller heart chambers and a fused atrio-ventricular canal were seen.
Specimen 1h LS Control: embryonic day 9.5 (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6456177).
Structure Level Pattern Image Note
TS15: atrium cardiac muscle Present 1h middle left
TS15: primitive ventricle cardiac muscle Present 1h middle left
Specimen 1h LS -/-: embryonic day 9.5; Bcas3tm1.1Msin/Bcas3tm1.1Msin (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6456177).
Structure Level Pattern Image Note
TS15: atrium cardiac muscle Present 1h middle right Collapsed, smaller heart chambers, reduced endocardium development and a fused atrio-ventricular canal were seen.
TS15: primitive ventricle cardiac muscle Present 1h middle right Collapsed, smaller heart chambers, reduced endocardium development and a fused atrio-ventricular canal were seen.
Specimen 5d E10.5 Control: embryonic day 10.5; Bcas3tm1Msin/? (more )
Note: The genotype of the control was not specified, either flox/flox, or flox/+.
Structure Level Pattern Image Note
TS17: atrium cardiac muscle Present 5d E10.5 Heart left
TS17: primitive ventricle cardiac muscle Present 5d E10.5 Heart left
Specimen 5d E10.5 CKO: embryonic day 10.5; Bcas3tm1Msin/Bcas3tm1Msin, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: atrium cardiac muscle Present 5d E10.5 Heart right Collapsed, smaller heart chambers and reduced endocardium development.
TS17: primitive ventricle cardiac muscle Present 5d E10.5 Heart right Collapsed, smaller heart chambers and reduced endocardium development.
Specimen 5d E11.5 Control: embryonic day 11.5; Bcas3tm1Msin/? (more )
Note: The genotype of the control was not specified, either flox/flox, or flox/+.
Structure Level Pattern Image Note
TS19: atrium cardiac muscle Present 5d E11.5 Heart left
TS19: ventricle cardiac muscle Present 5d E11.5 Heart left
Specimen 5d E11.5 CKO: embryonic day 11.5; Bcas3tm1Msin/Bcas3tm1Msin, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: atrium cardiac muscle Present 5d E11.5 Heart right Collapsed, smaller heart chambers and reduced endocardium development.
TS19: ventricle cardiac muscle Present 5d E11.5 Heart right Collapsed, smaller heart chambers and reduced endocardium development.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory