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Gene Expression Data
Assay Details
Assay
Reference: J:262744 Shetty R, et al., Rudhira/BCAS3 is essential for mouse development and cardiovascular patterning. Sci Rep. 2018 Apr 4;8(1):5632
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6456177
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1g Control: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: blood vessel Present 1g left Well formed vascular network comprising major vessels giving rise to intricate secondary and tertiary branches.
TS15: head blood vessel Present 1g head left Well formed vascular network comprising major vessels giving rise to intricate secondary and tertiary branches.
TS15: heart blood vessel Present 1g heart left Well formed vascular network comprising major vessels giving rise to intricate secondary and tertiary branches.
TS15: intersomitic blood vessel Present 1g ISV left Well formed vascular network comprising major vessels giving rise to intricate secondary and tertiary branches.
Specimen 1g -/-: embryonic day 9.5; Bcas3tm1.1Msin/Bcas3tm1.1Msin (more )
Structure Level Pattern Image Note
TS15: blood vessel Present 1g right Vasculature defects were seen.
TS15: head blood vessel Present 1g head right Completely disorganized head vasculature with defective vessel sprouting and reduced capillaries.
TS15: heart blood vessel Present 1g heart right Irregular and discontinuous vasculature.
TS15: intersomitic blood vessel Present 1g ISV right Irregular and discontinuous vasculature, and impaired branching that failed to sprout into fine capillaries.
Specimen 1h LS control: embryonic day 9.5 (more )
Note: Double labeled: red - Pecam1; green - Acta2 (assay MGI:6456236).
Structure Level Pattern Image Note
TS15: heart blood vessel Present 1h middle left
Specimen 1h LS -/-: embryonic day 9.5; Bcas3tm1.1Msin/Bcas3tm1.1Msin (more )
Note: Double labeled: red - Pecam1; green - Acta2 (assay MGI:6456236).
Structure Level Pattern Image Note
TS15: heart blood vessel Present 1h middle right Collapsed, smaller heart chambers, reduced endocardium development and a fused atrio-ventricular canal were seen.
Specimen 1h DA control: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: dorsal aorta Present Regionally restricted 1h lower left Expressed in the endothelial cells.
Specimen 1h DA -/-: embryonic day 9.5; Bcas3tm1.1Msin/Bcas3tm1.1Msin (more )
Structure Level Pattern Image Note
TS15: dorsal aorta Present Regionally restricted 1h lower right Expressed in the irregular and discontinuous endothelial cells with a pronounced decrease in the lumen.
Specimen 2a control: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present 2a left Finely patterned honey-comb like vascular network was seen.
Specimen 2a -/-: embryonic day 9.5; Bcas3tm1.1Msin/Bcas3tm1.1Msin (more )
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present 2a right Irregular and fused vascular network was seen.
Specimen 5d E10.5 DA control: embryonic day 10.5; Bcas3tm1Msin/? (more )
Note: The genotype of the control was not specified, either flox/flox, or flox/+.
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 5d E10.5 DA left Expressed in endothelial cells.
Specimen 5d E10.5 DA CKO: embryonic day 10.5; Bcas3tm1Msin/Bcas3tm1Msin, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 5d E10.5 DA right Expressed in the endothelial cells, although discontinuous and improperly patterned.
Specimen 5d E11.5 DA control: embryonic day 11.5; Bcas3tm1Msin/? (more )
Note: The genotype of the control was not specified, either flox/flox, or flox/+.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Regionally restricted 5d E11.5 DA left Expressed in the endothelial cells.
Specimen 5d E11.5 DA CKO: embryonic day 11.5; Bcas3tm1Msin/Bcas3tm1Msin, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: dorsal aorta Present Regionally restricted 5d E11.5 DA right Expressed in the endothelial cells, although discontinuous and improperly patterned.
Specimen 5d E10.5 ISV control: embryonic day 10.5; Bcas3tm1Msin/? (more )
Note: The genotype of the control was not specified, either flox/flox, or flox/+.
Structure Level Pattern Image Note
TS17: intersomitic blood vessel Present Regionally restricted 5d E10.5 ISV left Expressed in endothelial cells.
Specimen 5d E10.5 ISV CKO: embryonic day 10.5; Bcas3tm1Msin/Bcas3tm1Msin, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: intersomitic blood vessel Present Regionally restricted 5d E10.5 ISV right Expressed in the endothelial cells, although discontinuous and improperly patterned.
Specimen 5d E11.5 ISV control: embryonic day 11.5; Bcas3tm1Msin/? (more )
Note: The genotype of the control was not specified, either flox/flox, or flox/+.
Structure Level Pattern Image Note
TS19: intersomitic blood vessel Present Regionally restricted 5d E11.5 ISV left Expressed in endothelial cells.
Specimen 5d E11.5 ISV CKO: embryonic day 11.5; Bcas3tm1Msin/Bcas3tm1Msin, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: intersomitic blood vessel Present Regionally restricted 5d E11.5 ISV right Expressed in the endothelial cells, although discontinuous and improperly patterned.
Specimen 5e control: embryonic day 10.5; Bcas3tm1Msin/? (more )
Note: The genotype of the control was not specified, either flox/flox, or flox/+.
Structure Level Pattern Image Note
TS17: head blood vessel Present 5e left , 5e Head left Well formed vascular network comprising major vessels giving rise to intricate secondary and tertiary branches.
TS17: intersomitic blood vessel Present 5e ISV left , 5e left Well formed vascular network comprising major vessels giving rise to intricate secondary and tertiary branches.
Specimen 5e CKO: embryonic day 10.5; Bcas3tm1Msin/Bcas3tm1Msin, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: head blood vessel Present 5e right , 5e Head right Disorganized head vasculature with defective vessel sprouting.
TS17: intersomitic blood vessel Present 5e ISV right , 5e right Reduced capillaries and impaired branching that failed to sprout into fine capillaries.
Specimen 6a E10.5 Control: embryonic day 10.5; Bcas3tm1Msin/? (more )
Note: The genotype of the control was not specified, either flox/flox, or flox/+.
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present 6a upper left
Specimen 6a E10.5 CKO: embryonic day 10.5; Bcas3tm1Msin/Bcas3tm1Msin, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present 6a upper right Reduced branching from major vessels, vessel fusion and loss of branch hierarchy in the mutant.
Specimen 6a E11.5 Control: embryonic day 11.5; Bcas3tm1Msin/? (more )
Note: The genotype of the control was not specified, either flox/flox, or flox/+.
Structure Level Pattern Image Note
TS19: yolk sac blood vessel Present 6a lower left
Specimen 6a E11.5 CKO: embryonic day 11.5; Bcas3tm1Msin/Bcas3tm1Msin, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: yolk sac blood vessel Present 6a lower right Reduced branching from major vessels, vessel fusion and loss of branch hierarchy in the mutant.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory