Gene Expression Data
Assay Details
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Assay
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Reference:
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J:189955
Star EN, et al., Regulation of retinal interneuron subtype identity by the Iroquois homeobox gene Irx6. Development. 2012 Dec;139(24):4644-55
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6450178
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Gene symbol:
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Tacr3
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Gene name:
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tachykinin receptor 3
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Antibody:
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Anti-Nk3r
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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Two anti-Tacr3 antibodies were described in the methods Table S1. Either of the following could have been used: MGI:6450079 or MGI:6450081.
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Results
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Specimen
3A:
postnatal month 2-3; Irx6tm1Chow/Irx6 +
(more  )
Note: Double labeled: red - Tacr3; green - Irx6 (assay MGI:6450171).
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6+
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Tacr3; green - Irx6 (assay MGI:6450171).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
3C
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3A
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Tacr3 labels type 1 and 2 bipolar cells. Tacr3 co-labeled with Irx6:b-gal positive cells in a subset of bipolar cells.
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Specimen
5E:
postnatal month 2-3; Irx6tm1Chow/Irx6 +
(more )
Specimen
5E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6+
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6tm1Chow
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
5F
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Expression in type 1 and 2 bipolar cells was still present in the mutant retina.
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Specimen
9B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6+
Vsx1tm1Mci/Vsx1+
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tacr3; red - Hcn4 (assay MGI:6450186).
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Specimen
9E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6tm1Chow
Vsx1tm1Mci/Vsx1+
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tacr3; red - Hcn4 (assay MGI:6450186).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
9E
,
9F
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Ectopic Tacr3 expression was observed in Hcn4-immunolabeled cell somas of putative type 3a cells. Expression in type 1 and 2 bipolar cells was not co-labeled with Hcn4. There was an increased labeling of Tacr3 in sublamina 2.
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| TS28: retina inner plexiform layer |
Present |
Regionally restricted |
9E'
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9F'
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Ectopic Tacr3 expression was observed in Hcn4-immunolabeled putative type 3a axon terminals.
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Specimen
9H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
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Mutant Allele(s):
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Irx6tm1Chow/Irx6tm1Chow
Vsx1tm1Mci/Vsx1tm1Mci
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Age:
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postnatal month 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tacr3; red - Hcn4 (assay MGI:6450186).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
9I
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9H
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In the double mutant, all of the Hcn4-positive bipolar cells co-immunolabeled with Tacr3. There was a decrease of Tacr3-only immunolabeled cells.
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| TS28: retina inner plexiform layer |
Present |
Regionally restricted |
9H'
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9I'
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Hcn4 and Tacr3 co-immunolabeling was noted in the inner plexiform layer.
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