Pou5f1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:290529 Wallingford MC, et al., YY1 Is Required for Posttranscriptional Stability of SOX2 and OCT4 Proteins. Cell Reprogram. 2017 Aug;19(4):263-269
Assay type:	RNA in situ
MGI Accession ID:	MGI:6446760
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Probe:	Oct4 probe23
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 6G: embryonic day 7.5 (more

)
Note: Littermate control. Transverse section.

Structure	Level	Image	Note
TS11: embryo	Present	6G	Expression is seen in the epiblast.
TS11: neural ectoderm	Present	6G
TS11: primitive streak	Present	6G

Specimen 6I: embryonic day 7.5; Yy1^tm1Yshi/Yy1^tm2.1Yshi, Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺ (more

)
Note: Conditional mutant. Transverse section.

Structure	Level	Image	Note
TS11: embryo	Present	6I	Expression in the epiblast is (relatively) normal in the mutants.
TS11: neural ectoderm	Present	6I	Expression is (relatively) normal in the mutants.
TS11: primitive streak	Present	6I	Expression is (relatively) normal in the mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 12/30/2025 MGI 6.24