About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:290315 Cheong A, et al., Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation. Development. 2020 May 26;147(10):dev188714
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6445616
Gene symbol: Trp53
Gene name: transformation related protein 53
Antibody: anti-phospho-p53 (CS; 9284)
Detection system: Secondary antibody coupled to Alexa Fluor 546
Assay notes: The antibody used in this assay recognizes the active (phosphorylated) form of the protein.
Results
Specimen 4A: embryonic day 7.5 (more )
Note: Littermate control; embryo is either +/+ or +/-. Double labeled: white - Trp53; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS11: conceptus Absent 4A , 4A'' This protein is a marker of apoptosis; no dying cells were observed.
Specimen 4B: embryonic day 7.5; Mrpl3em1(IMPC)J/Mrpl3em1(IMPC)J (more )
Note: Double labeled: white - Trp53; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Absent 4B'' , 4B This protein is a marker of apoptosis; no dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
Specimen 4C: embryonic day 7.5; Mrpl22tm1b(KOMP)Mbp/Mrpl22tm1b(KOMP)Mbp (more )
Note: Double labeled: white - Trp53; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Present Single cells 4C'' , 4C This protein is a marker of apoptosis; a few dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
Specimen 4D: embryonic day 7.5; Mrpl44em1(IMPC)J/Mrpl44em1(IMPC)J (more )
Note: Double labeled: white - Trp53; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Present Single cells 4D , 4D'' This protein is a marker of apoptosis; a few dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
Specimen 4E: embryonic day 7.5; Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg (more )
Note: Double labeled: white - Trp53; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Present Single cells 4E'' , 4E This protein is a marker of apoptosis; a few dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
Specimen 4F: embryonic day 7.5; Mrps22tm1.1(KOMP)Vlcg/Mrps22tm1.1(KOMP)Vlcg (more )
Note: Double labeled: white - Trp53; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Absent 4F , 4F'' This protein is a marker of apoptosis; no dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
Specimen 4G: embryonic day 5.5 (more )
Note: Early egg-cylinder stage embryo. Double labeled: white - Trp53; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS7: conceptus Absent 4G'' , 4G This protein is a marker of apoptosis; no dying cells were observed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory