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Gene Expression Data
Assay Details
Assay
Reference: J:229352 Metzger DE, et al., Grg3/TLE3 and Grg1/TLE1 induce monohormonal pancreatic beta-cells while repressing alpha-cell functions. Diabetes. 2014 May;63(5):1804-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6441177
Gene symbol: Tle3
Gene name: transducin-like enhancer of split 3
Antibody: anti-Grg3
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A: embryonic day 17.5 (more )
Note: Triple labeled: green - Tle3; gray - Gcg (assay MGI:6441170); red - Ins1 (assay MGI:6441181) and Ins2 (assay MGI:6441475).
Structure Level Pattern Image Note
TS25: pancreas Present Regionally restricted 1A left , 1A middle , 1A right The majority of insulin-positive b-cells expressed Tle3. Many Gcg-positive alpha-cells were Tle3 negative or had low-level nuclear Tle3 expression.
Specimen 1B right: postnatal adult (more )
Note: Double labeled: green - Tle3; gray - Gcg (assay MGI:6441170).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present Regionally restricted 1B right , 1B middle right Many Gcg-positive alpha-cells were Tle3 negative or had low-level nuclear Tle3 expression. The percentage of Tle3+ alpha-cells decreased from E17.5 (47%) to the adult (14%).
Specimen 5A: embryonic day 15.5 (more )
Note: Double labeled: green - Tle3; red - Nkx6-1 (assay MGI:6441178).
Structure Level Pattern Image Note
TS23: pancreas Present 5A left , 5A right Coexpressed with Nkx6-1.
Specimen 5B: embryonic day 17.5 (more )
Note: Double labeled: green - Tle3; red - Nkx6-1 (assay MGI:6441178).
Structure Level Pattern Image Note
TS25: pancreas Present 5B left , 5B right Coexpressed with Nkx6-1.
Specimen 5C: postnatal newborn (more )
Note: Double labeled: green - Tle3; red - Nkx6-1 (assay MGI:6441178).
Structure Level Pattern Image Note
TS27: pancreas Present 5C right , 5C left Coexpressed with Nkx6-1.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory