Gene Expression Data
Assay Details
Assay
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Reference:
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J:222345
Rogers SW, et al., Nicotinic receptor Alpha7 expression during tooth morphogenesis reveals functional pleiotropy. PLoS One. 2012;7(5):e36467
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6433065
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Gene symbol:
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Chrna7
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Gene name:
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cholinergic receptor, nicotinic, alpha polypeptide 7
|
|
Results
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Specimen
1A:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1B:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
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Embedding:
|
Cryosection
|
Specimen
1C:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: molar mesenchyme |
Present |
Regionally restricted |
1C
|
Expressed in the condensing mesenchyme adjacent to epithelium underlying the primary enamel knot. Some weaker and inconsistent expression is detected in scattered cells that surround the primary enamel knot.
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Specimen
1D:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1E:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1F:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
2% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: molar enamel organ |
Present |
Regionally restricted |
1F
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Expressed in the molar ameloblasts.
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TS28: trigeminal nerve |
Present |
Regionally restricted |
1F
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Expressed in pioneering nerve fibers and some residual nerve fibers still surrounding the tooth organ.
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Specimen
1G:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
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Age:
|
postnatal day 7
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Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
2% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: molar enamel organ |
Present |
Regionally restricted |
1G
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Expressed in the molar ameloblasts and the enamel layer.
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TS28: trigeminal nerve |
Present |
Regionally restricted |
1G
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Expressed in the nerve fiber in the dental pulp.
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Specimen
1H:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
postnatal day 12
|
Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1I:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: incisor enamel organ |
Present |
Regionally restricted |
1I
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Expressed in the preameloblasts, starting in the medial aspect relative to the central axis.
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Specimen
1J:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
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Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
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Age:
|
postnatal day 0
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
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Embedding:
|
Cryosection
|
Specimen
1K:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
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Age:
|
postnatal day 12
|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
2% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
1L:
postnatal day 12; Chrna7tm1.1Swr/Chrna7 +
(more )
Note: Double labeled: green - Chrna7; red - Cux1 (assay MGI:6433101).
Specimen
1L:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
postnatal day 12
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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Double labeled: green - Chrna7; red - Cux1 (assay MGI:6433101).
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Specimen
2A:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: trigeminal nerve |
Present |
Regionally restricted |
2A
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Expressed in the pioneering processes of the molar nerve, extending to the buccal side of the dental mesenchyme.
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Specimen
2B:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: trigeminal nerve |
Present |
Regionally restricted |
2B
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Expressed in the prominent nerve net that surrounds developing molars.
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TS24: molar |
Absent |
|
2B
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Weak or essentially no expression in the structures of the developing tooth organ.
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Specimen
2C:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
postnatal day 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: trigeminal nerve |
Present |
Regionally restricted |
2C
|
Expressed in the pioneering fibers of the molar nerve, entering the tooth at the base of the molar dental papilla.
|
Specimen
2D:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
postnatal day 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: trigeminal nerve |
Present |
Regionally restricted |
2D
|
Expressed in the pioneering fibers of the molar nerve, entering the tooth at the base of the molar dental papilla at sites coincident with the location of blood vessels.
|
Specimen
2E:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: trigeminal nerve |
Present |
Regionally restricted |
2E
|
Expressed in molar nerves with a more extensive pattern as these processes were elaborated and became more widely distributed within the tooth pulp.
|
Specimen
2F:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
postnatal day 12
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: trigeminal nerve |
Present |
Regionally restricted |
2F
|
Expressed in an extensive array of molar nerve fibers that begin as aggregated tracts at the base of the dental papilla and then branch to form an extensive and dense fiber complex in the vicinity of the odontoblasts.
|
Specimen
2G:
postnatal day 12; Chrna7tm1.1Swr/Chrna7 +
(more )
Note: Double labeled: green - Chrna7; red - Tubb3 (assay MGI:6433100).
Specimen
2G:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
postnatal day 12
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Chrna7; red - Tubb3 (assay MGI:6433100).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: trigeminal nerve |
Present |
Regionally restricted |
2G
|
Expressed in an extensive array of molar nerve fibers. Some of them enter the odontoblast layer, although they do so sparingly.
|
Specimen
3A:
embryonic day 14.5; Chrna7tm1.1Swr/Chrna7 +
(more )
Note: Double labeled: green - Chrna7; red - Edar (assay MGI:6433099).
Specimen
3A:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Chrna7; red - Edar (assay MGI:6433099).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: molar mesenchyme |
Present |
Regionally restricted |
3A GFP
,
3A Merge
|
Expressed in the condensing mesenchyme immediately opposite the Edar+ primary enamel knot.
|
TS22: molar dental papilla |
Present |
Scattered |
3A Merge
,
3A GFP
|
Coexpressed with Edar in scattered cells in the dental papilla surrounding the mesenchyme. The majority of these cells have a classic spider-like morphology characteristic of macrophages.
|
Specimen
3B:
embryonic day 16.5; Chrna7tm1.1Swr/Chrna7 +
(more )
Note: Double labeled: green - Chrna7; red - Edar (assay MGI:6433099).
Specimen
3B:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Chrna7; red - Edar (assay MGI:6433099).
|
Specimen
5A:
postnatal day 10; Chrna7tm1.1Swr/Chrna7 +
(more )
Note: Double labeled: green - Chrna7; red - Chrna7 (assay MGI:6433098).
Specimen
5A:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Chrna7; red - Chrna7 (assay MGI:6433098).
|
Specimen
not shown:
embryonic day 13.5; Chrna7tm1.1Swr/Chrna7 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: trigeminal nerve |
Trace |
Regionally restricted |
|
Occasional fibers were inconsistently detected.
|
Specimen
not shown:
embryonic day 15.5; Chrna7tm1.1Swr/Chrna7 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: trigeminal nerve |
Present |
Regionally restricted |
|
Expressed in the pioneering processes of the molar nerve. The first bifurcations to the lingual aspect were seen.
|
TS23: molar mesenchyme |
Absent |
|
|
|
Specimen
not shown:
embryonic day 17.5; Chrna7tm1.1Swr/Chrna7 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
either: (B6.129-Chrna7tm1.1Swr) or (C3.129-Chrna7tm1.1Swr)
|
Mutant Allele(s):
|
Chrna7tm1.1Swr/Chrna7+
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|