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Gene Expression Data
Assay Details
Assay
Reference: J:222345 Rogers SW, et al., Nicotinic receptor Alpha7 expression during tooth morphogenesis reveals functional pleiotropy. PLoS One. 2012;7(5):e36467
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6433065
Gene symbol: Chrna7
Gene name: cholinergic receptor, nicotinic, alpha polypeptide 7
Reporter: GFP
Antibody: anti-GFP (Aves)
Detection system: fluorescein
Results
Specimen 1A: embryonic day 11.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS19: jaw primordium epithelium Present Regionally restricted 1A Expressed in the thickened dental lamina of the oral epithelium adjacent to or between developing tooth buds.
TS19: jaw primordium mesenchyme Trace Single cells 1A Occasionally expressed.

Specimen 1B: embryonic day 13.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS21: molar mesenchyme Present Scattered 1B
TS21: molar epithelium Absent 1B

Specimen 1C: embryonic day 14.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS22: molar mesenchyme Present Regionally restricted 1C Expressed in the condensing mesenchyme adjacent to epithelium underlying the primary enamel knot. Some weaker and inconsistent expression is detected in scattered cells that surround the primary enamel knot.

Specimen 1D: embryonic day 16.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS24: molar Absent 1D
TS24: trigeminal nerve Present 1D

Specimen 1E: embryonic day 18.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS26: molar enamel organ Present Regionally restricted 1E Expressed in the molar ameloblasts.

Specimen 1F: postnatal day 4; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 1F Expressed in the molar ameloblasts.
TS28: trigeminal nerve Present Regionally restricted 1F Expressed in pioneering nerve fibers and some residual nerve fibers still surrounding the tooth organ.

Specimen 1G: postnatal day 7; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 1G Expressed in the molar ameloblasts and the enamel layer.
TS28: trigeminal nerve Present Regionally restricted 1G Expressed in the nerve fiber in the dental pulp.

Specimen 1H: postnatal day 12; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present 1H Decreased expression.
TS28: trigeminal nerve Present Regionally restricted 1H Expressed in the nerve fiber in the dental pulp.

Specimen 1I: embryonic day 16.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS24: incisor enamel organ Present Regionally restricted 1I Expressed in the preameloblasts, starting in the medial aspect relative to the central axis.

Specimen 1J: postnatal day 0; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS27: incisor enamel organ Strong Regionally restricted 1J Expressed in the ameloblasts.

Specimen 1K: postnatal day 12; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS28: incisor enamel organ Present Regionally restricted 1K Expressed in the ameloblasts.

Specimen 1L: postnatal day 12; Chrna7tm1.1Swr/Chrna7+ (more )
Note: Double labeled: green - Chrna7; red - Cux1 (assay MGI:6433101).
Structure Level Pattern Image Note
TS28: incisor enamel organ Present Regionally restricted 1L Expressed in the ameloblasts.
TS28: incisor dental pulp Absent 1L

Specimen 2A: embryonic day 14.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS22: trigeminal nerve Present Regionally restricted 2A Expressed in the pioneering processes of the molar nerve, extending to the buccal side of the dental mesenchyme.

Specimen 2B: embryonic day 16.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS24: trigeminal nerve Present Regionally restricted 2B Expressed in the prominent nerve net that surrounds developing molars.
TS24: molar Absent 2B Weak or essentially no expression in the structures of the developing tooth organ.

Specimen 2C: postnatal day 4; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS28: trigeminal nerve Present Regionally restricted 2C Expressed in the pioneering fibers of the molar nerve, entering the tooth at the base of the molar dental papilla.

Specimen 2D: postnatal day 4; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS28: trigeminal nerve Present Regionally restricted 2D Expressed in the pioneering fibers of the molar nerve, entering the tooth at the base of the molar dental papilla at sites coincident with the location of blood vessels.

Specimen 2E: postnatal day 6; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS28: trigeminal nerve Present Regionally restricted 2E Expressed in molar nerves with a more extensive pattern as these processes were elaborated and became more widely distributed within the tooth pulp.

Specimen 2F: postnatal day 12; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS28: trigeminal nerve Present Regionally restricted 2F Expressed in an extensive array of molar nerve fibers that begin as aggregated tracts at the base of the dental papilla and then branch to form an extensive and dense fiber complex in the vicinity of the odontoblasts.

Specimen 2G: postnatal day 12; Chrna7tm1.1Swr/Chrna7+ (more )
Note: Double labeled: green - Chrna7; red - Tubb3 (assay MGI:6433100).
Structure Level Pattern Image Note
TS28: trigeminal nerve Present Regionally restricted 2G Expressed in an extensive array of molar nerve fibers. Some of them enter the odontoblast layer, although they do so sparingly.

Specimen 3A: embryonic day 14.5; Chrna7tm1.1Swr/Chrna7+ (more )
Note: Double labeled: green - Chrna7; red - Edar (assay MGI:6433099).
Structure Level Pattern Image Note
TS22: molar mesenchyme Present Regionally restricted 3A GFP , 3A Merge Expressed in the condensing mesenchyme immediately opposite the Edar+ primary enamel knot.
TS22: molar dental papilla Present Scattered 3A Merge , 3A GFP Coexpressed with Edar in scattered cells in the dental papilla surrounding the mesenchyme. The majority of these cells have a classic spider-like morphology characteristic of macrophages.

Specimen 3B: embryonic day 16.5; Chrna7tm1.1Swr/Chrna7+ (more )
Note: Double labeled: green - Chrna7; red - Edar (assay MGI:6433099).
Structure Level Pattern Image Note
TS24: molar Absent 3B Merge , 3B GFP

Specimen 5A: postnatal day 10; Chrna7tm1.1Swr/Chrna7+ (more )
Note: Double labeled: green - Chrna7; red - Chrna7 (assay MGI:6433098).
Structure Level Pattern Image Note
TS28: incisor enamel organ Present Regionally restricted 5A right , 5A left Expressed in the ameloblasts, essentially the entire cell, except for the nucleus.

Specimen not shown: embryonic day 13.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS21: trigeminal nerve Trace Regionally restricted Occasional fibers were inconsistently detected.

Specimen not shown: embryonic day 15.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS23: trigeminal nerve Present Regionally restricted Expressed in the pioneering processes of the molar nerve. The first bifurcations to the lingual aspect were seen.
TS23: molar mesenchyme Absent

Specimen not shown: embryonic day 17.5; Chrna7tm1.1Swr/Chrna7+ (more )
Structure Level Pattern Image Note
TS25: incisor enamel organ Present Widespread Expressed in all ameloblasts.


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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory