Ric8a Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:271730 Kask K, et al., Targeted deletion of RIC8A in mouse neural precursor cells interferes with the development of the brain, eyes, and muscles. Dev Neurobiol. 2018 Apr;78(4):374-390
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6416420
Gene symbol:	Ric8a
Gene name:	RIC8 guanine nucleotide exchange factor A

Antibody:	anti-RIC8A clone 3G3 (ab118151)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

Specimen 1B: embryonic day 14.5 (more

)
Note: Littermate control; the genotype was either NestinCre+/-Ric8a+/^tm2.1Aks, NestinCre-/-;Ric8a^tm1Aks/^tm2.1Aks, or NestinCre-/-; Ric8a+/^tm2.1Aks. Double labeled: red - Ric8a; green - laminin.

Structure	Level	Pattern	Image	Note
TS22: telencephalon subarachnoid space	Present	Regionally restricted	1B , 1C , 1D	Expression is in arachnoid trabeculae.
TS22: cerebral cortex	Present		1D , 1C , 1B
TS22: leptomeninges	Present		1D , 1C , 1B
TS22: telencephalon dura mater	Present		1D , 1C , 1B
TS22: telencephalon pia mater	Present		1D , 1C , 1B

Specimen 1B': embryonic day 14.5; Ric8a^tm1Aks/Ric8a^tm2.1Aks, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Double labeled: red - Ric8a; green - laminin.

Structure	Level	Pattern	Image	Note
TS22: telencephalon subarachnoid space	Weak		1D' , 1B' , 1C'	There is a diminished arachnoid trabeculae network in this mutant.
TS22: telencephalon dura mater	Weak	Regionally restricted	1C' , 1B' , 1D'
TS22: cerebral cortex	Absent		1D' , 1B' , 1C'
TS22: telencephalon pia mater	Absent		1D' , 1B' , 1C'

Specimen 2A: embryonic day 14.5 (more

)
Note: Littermate control; the genotype was either NestinCre+/-Ric8a+/^tm2.1Aks, NestinCre-/-;Ric8a^tm1Aks/^tm2.1Aks, or NestinCre-/-; Ric8a+/^tm2.1Aks. Double labeled: red - Ric8a; green - Calb2 (assay MGI:6416424).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2A , 2B	Expression is absent in Cajal-Retzius cells.

Specimen 2A': embryonic day 14.5; Ric8a^tm1Aks/Ric8a^tm2.1Aks, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Double labeled: red - Ric8a; green - Calb2 (assay MGI:6416424).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Absent		2B' , 2A'

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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