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Gene Expression Data
Assay Details
Assay
Reference: J:221356 Snowball J, et al., Mesenchymal Wnt signaling promotes formation of sternum and thoracic body wall. Dev Biol. 2015 May 15;401(2):264-75
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6407008
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Results
Specimen 2G: embryonic day 12.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: red - Acta2; blue - Sox9 (assay MGI:6406997); green - Cre reporter.
Structure Level Pattern Image Note
TS20: body wall Present Regionally restricted 2G Expression in ventral body wall.
Specimen 2H: embryonic day 12.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: red - Acta2; blue - Sox9 (assay MGI:6406997); green - Cre reporter.
Structure Level Pattern Image Note
TS20: body wall Present Regionally restricted 2H , 2H' Expression in ventral body wall.
Specimen 4B Control: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present 4B Control Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen 4B Wls f/f; Dermo1 Cre/+: embryonic day 14.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present 4B Wls f/f; Dermo1 Cre/+ Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen 4C Control: embryonic day 14.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: thoracic body wall Present Regionally restricted 4C Control Expression between sternal bars.
Specimen 4C Wls f/f; Dermo1 Cre/+: embryonic day 14.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present 4C Wls f/f; Dermo1 Cre/+
Specimen 7C Control (upper): embryonic day 14.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present Regionally restricted 7C Control (upper) Expression in myocytes appearing as leading cells between almost fused sternal bars.
Specimen 7C Wls f/f; Dermo1 Cre/+: embryonic day 14.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: thoracic body wall Present Regionally restricted 7C Wls f/f; Dermo1 Cre/+ Expression in poorly organized cells in ventral body wall.
Specimen 7C Control (lower): embryonic day 14.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: pectoral girdle and thoracic body wall musculature Present Regionally restricted 7C Control (lower) Expression in myocytes appearing as leading cells between almost fused sternal bars.
Specimen 7C LRP5/6 f/f; Dermo1 Cre/+: embryonic day 14.5; Lrp5tm1.1Vari/Lrp5tm1.1Vari, Lrp6tm1.1Vari/Lrp6tm1.1Vari, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Sox9 (assay MGI:6406997).
Structure Level Pattern Image Note
TS22: thoracic body wall Present Regionally restricted 7C LRP5/6 f/f; Dermo1 Cre/+ Expression in poorly organized cells in ventral body wall.
Specimen S3B Control (1st row): embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Control (1st row)
Specimen S3B Wls f/f; Dermo1 Cre/+ (1st row): embryonic day 13.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Wls f/f; Dermo1 Cre/+ (1st row) Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen S3B Control BW (2nd row): embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Control (2nd row)
Specimen S3B Wls f/f; Dermo1 Cre/+ BW (2nd row): embryonic day 13.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Wls f/f; Dermo1 Cre/+ (2nd row) Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen Double labeled: red - Acta2; blue - DAPI.S3B Control BW (3rd row): embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Control (3rd row)
Specimen S3B Wls f/f; Dermo1 Cre/+ BW (3rd row): embryonic day 13.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Wls f/f; Dermo1 Cre/+ (3rd row) Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen S3B Control Fl (4th row): embryonic day 13.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Control (4th row)
Specimen S3B Wls f/f; Dermo1 Cre/+ Fl (4th row): embryonic day 13.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS21: pectoral girdle and thoracic body wall musculature Present S3B Wls f/f; Dermo1 Cre/+ (4th row) Authors report expression in skeletal and smooth muscle was similar to that of controls.
Specimen S4 Control (1st row left): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control (top row left)
Specimen S4 Wls f/f;Dermo1 Cre/+ (1st row): embryonic day 11.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Wls f/f; Dermo1 Cre/+ (top row)
Specimen S4 Control (2nd row left): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control Body wall (left)
Specimen S4 Wls f/f;Dermo1 Cre/+ (2nd row): embryonic day 11.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Wls f/f; Dermo1 Cre/+ Body wall
Specimen S4 Control (3rd row left): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control Flanks (left)
Specimen S4 Wls f/f;Dermo1 Cre/+ (3rd row): embryonic day 11.5; Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Wls f/f; Dermo1 Cre/+ Flanks
Specimen S4 Control (1st row right): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control (top row right)
Specimen S4 LRP5/6 f/f Dermo1 Cre/+ (1st row): embryonic day 11.5; Lrp5tm1.1Vari/Lrp5tm1.1Vari, Lrp6tm1.1Vari/Lrp6tm1.1Vari, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_LRP5/6 f/f; Dermo1 Cre/+ (top row)
Specimen S4 Control (2nd row right): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control Body wall (right)
Specimen S4 LRP5/6 f/f Dermo1 Cre/+ (2nd row): embryonic day 11.5; Lrp5tm1.1Vari/Lrp5tm1.1Vari, Lrp6tm1.1Vari/Lrp6tm1.1Vari, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_LRP5/6 f/f; Dermo1 Cre/+ Body wall
Specimen S4 Control (3rd row right): embryonic day 11.5 (more )
Note: Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_Control Flanks (right)
Specimen S4 LRP5/6 f/f Dermo1 Cre/+ (3rd row): embryonic day 11.5; Lrp5tm1.1Vari/Lrp5tm1.1Vari, Lrp6tm1.1Vari/Lrp6tm1.1Vari, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; white - BrdU.
Structure Level Pattern Image Note
TS19: body wall Present S4_LRP5/6 f/f; Dermo1 Cre/+ Flanks
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